magistrsko delo
Natalija Virant (Author), Mitja Kolar (Mentor), Darinka Brodnjak-Vončina (Co-mentor), Karin Stana-Kleinschek (Co-mentor)

Abstract

Namen magistrskega dela je bil razviti in validirati HPLC analizno metodo za določanje sproščanja benzokaina iz nanovlaken. V ta namen smo pripravili elektropredena nanovlakna z benzokainom in optimizirali postopek elektropredenja iz celuloznega acetata (CA). Pri tem smo preučevali vpliv procesnih parametrov in parametrov predilne raztopine na potek elektropredenja. Dobljena nanovlakna smo okarakterizirali s SEM in FTIR analizo, določili smo tudi stopnjo nabrekanja in stični kot. Koncentracije benzokaina smo določali z uporabo kolone Supelcosil LC-18 (20 cm x 4,6 cm, 3µm) in UV-VIS detektorja pri valovni dolžini 285 nm. Kot optimalno mobilno fazo smo izbrali acetonitril:voda (60:40) pri pretoku 0,8 ml/min. Pri teh pogojih se je benzokain eluiral pri 4,26 min. Določili smo, da je razvita metoda natančna, točna in linearna v območju med 5 in 50 mg/l. Meja zaznavnosti (LOD) znaša 1,08 mg/l, meja določljivosti (LOQ) pa 3,27 mg/l. Gladka nanovlakna, s premerom med 400 in 900 nm, smo izpredli iz 17 ut. % CA v 85 % ocetni kislini. Optimalni procesni parametri elektropredenja so: napetost 75 kV in razdalja med elektrodama 160 mm. Optimalni predilni raztopini smo dodali 5 % benzokaina (glede na maso CA) in ta dodatek ni vplival na postopek elektropredenja.

Keywords

tekočinska kromatografija visoke ločljivosti;validacija;elektropredenje;benzokain;kinetika sproščanja;adsorpcija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [N. Virant]
UDC: 543.544.5(043.2)
COBISS: 19035670 Link will open in a new window
Views: 3061
Downloads: 272
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Other data

Secondary language: English
Secondary title: DEVELOPMENT OF HPLC METHOD FOR DETERMINATION OF VARIOUS PHARMACEUTICAL COMPOUNDS AND STUDY OF SUPPORTING MATERIAL EROSION
Secondary abstract: The aim of the master thesis was developed and validated HPLC analysis method for determination of benzocaine release from nanofibers. For this purpose we have prepared electrospun nanofibers with benzocaine and optimized electrospinning process of cellulose acetate (CA). We have studied the influence of solution parameters and process parameters of electrospinning. Electrospun nanofibers were characterized by SEM and FTIR analysis. Swelling behaviors and contact angle were also investigated. Concentrations of benzocaine were determined using Supelcosil LC-18 (20 cm x 4,6 cm, 3µm) column. The UV-VIS detector was set at 285 nm. The mobile phase consisted of acetonitrile:water (60:40) and used flow rate was 0,8 ml/min. Under these conditions benzocaine eluted at 4,26 min. We confirmed that method was precise, accurate and linear from 5 to 50 mg/l. The limits of detection (LOD) and quantification (LOQ) values were 1,08 mg/l and 3,27 mg/l, respectively. Smooth nanofibers with diameters ranging from 400 to 900 nm were electrospun from a 17 ut. % CA solution in 85 % acetic acid. Optimal electrospinning parameters are 160 mm distance between electrodes and 75 kV voltage. The addition of 5 % benzocaine (based on the weight of CA) in the spinning formulation did not affect the fiber formation.
Secondary keywords: high performance liquid chromatography;HPLC;validation;electrospinning;benzocaine;drug release kinetics;
URN: URN:SI:UM:
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: XI, 68 str.
ID: 8966376