Supercritical fluid chromatography and scale up study

Miha Oman (Author), Petra Kotnik (Author), Mojca Škerget (Author), Željko Knez (Author)

Abstract

The influence of process parameters on supercritical fluid chromatography (SFC) and scale-up on to the preparative scale was investigated. In this scope, dependency of pressure, temperature, type and concentration of modifier, and type of stationary phase on the separation were examined separately. Experiments were performed on silica stationary phases in the range of pressures from 100 bar to 250 bar and temperatures from 30°C to 65°C, with use of ethanol and methanol as modifier. Results obtained on analytical scale were used for scale up on preparative (to production) scale. The aim of this study is presentation of the state-of-the-art of SFC through separate effects of process parameters on the separation and investigation of development, benefits and drawbacks of scaling-up process of the SFC.

Keywords

supercritical fluids;supercritical fluid chromatograpy;chromatography;scale up study;SFC;

Data

Language:	English
Year of publishing:	2014
Typology:	1.01 - Original Scientific Article
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC:	543.544.3/.5:54-139
COBISS:	18308886
ISSN:	1318-0207
Views:	1039
Downloads:	111
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary abstract:	Raziskali smo vpliv procesnih parametrov na kromatografijo s superkritičnimi fluidi (SFC) skupaj z povečevanjem obsega na preparativno merilo. V tem okviru smo posamezno raziskali odvisnost tlaka, temperature, tipa in koncentracije so-topila, ter tipa stacionarne faze na separacijo testnih komponent (kofeina in teofilina) s SFC. Eksperimente smo izvedli na napravah za SFC v analitičnem in pilotnem preparativnem merilu. Separacije smo izvajali na dveh stacionarnih fazah: čisti silica in silica 2-etilpiridina. Kot mobilno fazo smo uporabili $CO_2$/metanol ali $CO_2$/etanol pri povišanih tlakih in temperaturah. Rezultate eksperimentov iz SFC naprave v analitičnem merilu smo uporabili za povečevanje obsega na preparativno merilo. Cilj študije je bila evaluacija vpliva procesnih parametrov na separacijo testnih komponent in praktični prikaz povečevanja obsega in obratovanja pilotne preparativne naprave, ki sodi med večje podobne naprave v globalnem merilu.
Secondary keywords:	superkritični fluidi;kromatografija s superkritičnimi fluidi;superkritična kromatografija;separacijski procesi;
URN:	URN:NBN:SI
Type (COBISS):	Scientific work
Pages:	str. 746-758
Volume:	ǂVol. ǂ61
Issue:	ǂno. ǂ4
Chronology:	2014
ID:	9097644