diplomsko delo univerzitetnega študijskega programa I. stopnje
Nika Kodba (Author), Maja Leitgeb (Mentor), Mateja Primožič (Co-mentor)

Abstract

Diplomsko delo je sestavljeno iz treh delov. Prvi del zajema pripravo trdnega gojišča za rast glive Pleurotus ostreatus. Kot trdnen substrat smo uporabili pšenične otrobe treh velikosti, katere smo dobili s pomočjo sejalne analize. Prav tako, smo izvedli test za določitev vlažnosti substrata in mu določili optimalno 80 % vlažnost. V drugem delu gojili glivo na trdnem substratu in spremljanje prirasti glive P. ostreatus v odvisnosti od velikosti delcev pšeničnih otrobov in časa inkubacije. V zadnjem delu pa smo določali encimske aktivnosti iz ekstraktov micelija glive P. ostreatus. Zanimal nas je vpliv velikosti pšeničnih otrobov in čas inkubacije na aktivnost encima celulaza in lakaza. Aktivnosti za oba encima smo določali po 8., 11. in 14. dnevu inkubacije. Rezultati kažejo, da so aktivnosti za encim celulaza najvišje po 8. dneh inkubacije, za encim lakaza pa po 14. dneh. Vpliv velikosti zrnatosti pšeničnih otrobov na aktivnost encima lakaza predpisujemo gojišču iz srednje velikih otrobov in gojiščema iz velikih in srednje velikih otrobov za encim celulaza.

Keywords

Pleurotus ostreatus;pšenični otrobi;celulaza;lakaza;sejalna analiza;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [N. Kodba]
UDC: 664.764(043.2)
COBISS: 20304150 Link will open in a new window
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Downloads: 149
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Other data

Secondary language: English
Secondary title: INFLUENCE OF WHEAT BRAN PARTICLE SIZE ON GROWTH OF PLEUROTUS OSTREATUS FUNGI
Secondary abstract: The thesis consists of three parts. The first part covers the preparation of a solid breeding ground for growth of the fungus Pleurotus ostreatus. As a solid substrate we used three different sizes of wheat, which were obtained by using sieve analysis. We also carried out the test for determining the humidity of the substrate on which we defined the optimal 80% humidity. In the second part we carried out the cultivation of fungi on a solid substrate, and monitored the weight gain of the fungus P. ostreatus depending on the particle size of wheat bran and the time of incubation. In the last part we have determined the enzymatic activity of extracts from the mycelium of the fungus P. ostreatus. We were interested in the influence of the size of wheat bran and incubation time on the activity of the enzyme cellulase and laccase. Activities for both the enzymes were determined in the 8th, 11th and 14th day of incubation. The results show that the activity of the enzyme cellulase reaches it’s maximum after 8 days of incubation, the enzyme laccase after 14 days. The impact of the grain size of wheat bran on the activity of the enzyme laccase are attributed to the breeding grounds of medium sized bran, medium large and large sized bran for the enzyme cellulase.
Secondary keywords: wheat bran;cellulase;laccase;sieve analysis;
URN: URN:SI:UM:
Type (COBISS): Bachelor thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: IX, 30 str.
ID: 9150377
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