Priprava magnetnih zamreženih encimskih skupkov iz encima celulaza

magistrsko delo

Dušica Ifko (Author), Maja Leitgeb (Mentor)

Abstract

Namen magistrskega dela je določiti vpliv posameznih sinteznih parametrov na fizikalne in kemijske lastnosti zamreženih encimskih skupkov in magnetnih zamreženih encimskih skupkov iz encima celulaze in narediti primerjavo med CLEAs in mCLEAs. Iz encima celulaze smo vzporedno pripravili zamrežene encimske skupke in magnetne zamrežene encimske skupke in izvedli optimizacijo parametrov, ki vplivajo na aktivnost, učinkovitost in stabilnost imobilizacije. Preučevali smo vpliv obarjalnih reagentov, mrežnih povezovalcev, vpliv koncentracije glutaraladehida v kombinaciji z različnimi obarjalnimi reagenti na aktivnost in učinkovitost CLEAs in mCLEAs, temperaturo zamreženja, vpliv koncentracije encima, vpliv dodatkov in izpiranja z puferno raztopino ter vpliv koncentracije natrijevega cianoborohidrida na aktivnost in stabilnost imobilizacije. Uspešno smo sintetizirali celulazno CLEAs in mCLEAs, dobljene relativne aktivnosti za CLEAs so 86% in za mCLEAs 91%. Končna aktivnost po spiranju z vodo je večja za mCLEAs kot za CLEAs, ker imajo amino-funkcionalizirani magnetni nano delci dodatno povezovalno vlogo v postopku zamreženja. Izvedene so meritve stabilnosti na različnih temperaturah in možnost ponovne uporabe encima.

Keywords

celulaza;zamreženi encimski skupki;magnetni zamreženi encimski skupki;magnetni nanodelci maghemita;mrežni povezovalci;magistrske naloge;

Data

Language:	Slovenian
Year of publishing:	2016
Typology:	2.09 - Master's Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[D. Ifko]
UDC:	577.151(043)
COBISS:	19876374
Views:	1158
Downloads:	129
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	PREPARATION OF MAGNETIC CROO-LINKED ENZYME AGGREGATES OF THE CELLULASE
Secondary abstract:	The aim of this graduate thesis is to determine the influence of the synthesis parameters, such as different precipitants, additives, cross-linker and other parameters on the physical and chemical characteristics of cross-linked enzyme aggregates and magnetic cross-linked enzyme aggregates of the enzyme cellulase and comparisson of the characteristics of CLEAs and mCLEAs. The cross-linked enzyme aggregates CLEAs and magnetic cross-linked enzyme aggregates mCLEAs were prepared of the enzyme cellulase and optimization of the various parameters were performed paralelly. The effect of different precipitants, cross-linking agents, the impact of concentration glutaraldehyde in combination with a different precipitants to the activity and immobilisation efficiency of CLEAs and mCLEAs, the temperature of cross-linking, the effect of the concentration of the enzyme, the effect of additives, buffer washing, the influence of the concentration of sodium cyanoborohydride to the activity and stability were investigated. The cellulase CLEAs and mCLEAs were prepared successfully, relative activities for CLEAs 86% and 91% for mCLEAs were obtained. The final activity is higher for mCLEAs because of presence the amino-functionalized magnetic nanoparticles. Measurements of stability at different temperatures and reusability of the CLEAs and mCLEAs were performed.
Secondary keywords:	cellulase;cross-linked enzyme aggregates;magnetic cross-linked enzyme aggregates;maghemite magnetic nanoparticles;
Type (COBISS):	Master's thesis
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	X, 58 str.
ID:	9155183