Abstract
The optimum reaction parameters for the interaction of hexavalent chromium [Cr(VI)] with diphenylcarbazide in microfluidic
chips (μFIA) with thermal-lens microscopic detection were selected. The characteristic feature of the applied
flow scheme is the injection of the reagent into the stream containing the test metal, which enables in-field and
real-time monitoring of Cr(VI) simply by flowing the sample continuously through the microchip. The limit of detection
of Cr(VI) under the selected conditions (signal generating wavelength, 514.5 nm; excitation power, 100 mW; detection
position, 10 cm downstream from the mixing zone of the microchip; flow rate 10 μL min–1; injection volume,
1.4 μL) is 15 ng mL–1 (2.9 × 10–7 mol L–1). The linear range is 40 ng mL–1 – 10 μg mL–1 with a relative standard deviation
no higher than 10% in the concentration range 0.1–1 μg mL–1. The online monitoring by this scheme provides the
possibility of up to 360 analyses per hour.
Keywords
photothermal lensing;thermal-lens microscopic detection;microfluidic applications;hexavalent chromium;diphenylcarbazide;
Data
Language: |
English |
Year of publishing: |
2016 |
Typology: |
1.01 - Original Scientific Article |
Organization: |
UNG - University of Nova Gorica |
UDC: |
543 |
COBISS: |
4602875
|
ISSN: |
1318-0207 |
Views: |
3723 |
Downloads: |
0 |
Average score: |
0 (0 votes) |
Metadata: |
|
Other data
Secondary language: |
Slovenian |
Secondary title: |
Določevanje sledov Cr(VI) z difenilkarbazidom in μFIA–TLS mikroskopijo |
Secondary abstract: |
Za dolo~anje {estvalentnega kroma [Cr(VI)] s preto~no injekcijsko analizo v mikrofluidnem sistemu (μFIA) in detekcijo
z mikroskopijo na osnovi toplotnih le~, smo izbrali optimalne reakcijske pogoje za interakcijo Cr(VI) s
kolorimetri~nim reagentom difenilkarbazidom. Glavna zna~ilnost uporabljenega preto~nega sistema je injiciranje
reagenta v nosilni tok, ki vsebuje analit. To, ob stalnem pretoku vzorca skozi mikro~ip, v praksi omogo~a kontinuirni
monitoring Cr(VI) v realnem ~asu. Pri izbranih pogojih (vzbujevalna valovna dol`ina 514,5 nm, mo~ vzbujanja 100
mW, to~ka detekcije 10 cm po to~ki me{anja, pretok 10 μL min–1, injicirani volumen 1,4 μL) smo dosegli mejo zaznave
15 ng mL–1 (2,9 × 10–7 mol L–1). Pri tem je bilo obmo~je linearnosti 40 ng mL–1 – 10 μg mL–1, relativni standardni
odmik v koncentracijskem obmo~ju 0.1–1 μg mL–1 pa manj{i od 10 %. Opisani sistem omogo~a kontinuirno merjenje
koncentracije Cr(VI) s 360 analizami na uro. |
URN: |
URN:SI:UNG |
Type (COBISS): |
Not categorized |
Pages: |
str. 772-780 |
Volume: |
ǂVol. ǂ63 |
Issue: |
ǂno. ǂ4 |
Chronology: |
2016 |
DOI: |
10.17344/acsi.2016.2549 |
ID: |
9236759 |