Influence of transient response of platinum electrode on neural signals during stimulation of isolated swinish left vagus nerve

Polona Pečlin (Author), Franci Vode (Author), Andraž Mehle (Author), Igor Grešovnik (Author), Janez Rozman (Author)

Abstract

The main aim of the work was to measure transient response characteristics of interface between platinum stimulating electrodes and isolated swinish left cervical vagus nerve (segment), when electrical stimulating pulses are appliedto preselected locations along the segment and elicited neural signals,also described as compound action potentials (CAPs), are recorded fromparticular compartments of the nerve. The stimulating system was manufactured as a silicone self-coiling spiral cuff (cuff) with embedded matrix of ninety-nine rectangular electrodes (0.5 mm in width and 2mm in length), made of 45 microm thick annealed platinum ribbon (99.99 % purity), and a geometric surface of 1 mm2. For electrical stimulation, a current quasitrapezoidal, asymmetric and biphasic pulses with frequency of 1 Hz, were used. To test an influence of stimulating pulses having different parameters and waveforms on elicited CAPs, various degree of imbalance between an electric charge (charge) injected in cathodic phase as well as charge injectedin anodic phase of a biphasic stimulating pulse, were deployed and compared. To identify the differences in elicited CAPs however, an integral ofthe CAP cathodic phase as well as integral of the CAP anodic phase of stimulating pulse, were calculated and compared. Results showed a strong component superimposed in the CAPs, considered as an ensemble artefact which greatly obscured the components of the CAPs, and various components did overlap. Results also showed that stimulating pulses, having preset certain degree of imbalance between charge injected in cathodic and charge injected inanodic phase, elicited a slight change in a positive waveform deflection of CAP manifested under a cathodic phase as well as slight change in a negative waveform deflection of CAP manifested under an anodic phase. (Abstract truncated at 2000 characters)

Keywords

električna stimulacija;živec;platinasta elektroda;

Data

Language:	English
Year of publishing:	2012
Typology:	1.01 - Original Scientific Article
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	Inštitut za kovinske materiale in tehnologije Ljubljana
UDC:	616.8
COBISS:	29830361
ISSN:	1580-2949
Parent publication:	Materiali in tehnologije
Views:	921
Downloads:	91
Average score:	0 (0 votes)
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Other data

Secondary language:	Slovenian
Secondary title:	Vpliv prehodnega značaja platinaste elektrode na živčni signal med stimulacijo izoliranega živca vagusa svinje
Secondary abstract:	Glavni namen dela je bil izmeriti prehodni značaj na prehodu med platinasto stimulacijsko elektrodo in delom izoliranega levega vratnega prašičjega živca vagusa (segment) med dovajanjem stimulacijskih impulzov na izbrana mesta vzdolž živca in hkratnim merjenjem živčnega signala, imenovanega sestavljeni akcijski potencial (CAP) na določenih predelih živca. Stimulacijski sistem je bil izdelan v obliki silikonske spiralne objemke (cuff) z vdelano matriko devetindevetdestih pravokotnih elektrod (širina 0,5 mm in dolžina 2 mm), izdelanih iz žarjenega platinastega traku (čistost 99,99 %) in geometrijsko površino 1mm2. Za električno stimulacijo so bili uporabljeni tokovni kvazitrapezni, asimetrični in izmenični impulzi frekvence 1 Hz. Za preizkušanje vpliva stimulacijskih impulzov z različnimi parametri in oblikami na izzvani CAP je bila med katodno in anodno vneseni električni naboj uvedena določena stopnja neuravnoteženosti. Katodno in anodno vnesena naboja sta bila za izbrane impulze med seboj primerjana. S ciljem ugotavljanja razlik v izzvanem CAP-u pa sta bila izračunana in med seboj primerjana integrala tako CAP-a, prisotnega pod katodno fazo, kot CAP-a, prisotnega pod anodno fazo zgoraj omenjenega stimulacijskega impulza. Rezultati so pokazali močno komponento, položeno na CAP, ki je sestavljena motnja in ki znatno zamegli ter prekrije posamezne komponente CAP-a. Rezultati so tudi pokazali, da zgoraj omenjeni stimulacijski impulzi s prednastavljeno določeno stopnjo neravnotežja med nabojem, vnesenim v katodni fazi, ter nabojem, vnesenim v anodni fazi, izzovejo majhne spremembe pri pozitivnem odklonu CAP-a, prisotnega pod katodno fazo, kakor tudi majhne spremembe v negativnem odklonu CAP-a, prisotnega pod anodno fazo. Nadalje so bile opažene tudi majhne razlike v CAP-ih, izražene kot integral pod katodnim pozitivnim odklonom in kot integral pod anodnim negativnim odklonom. Končno je mogoče skleniti, da neravnotežje med katodno in anodno vnesenim nabojem ni znatno vplivalo na izmerjene CAP-e.
Secondary keywords:	električna stimulacija;živec;platinasta elektroda;levi živec vagus;elektrokemija;električni naboj;
URN:	URN:NBN:SI
Type (COBISS):	Scientific work
Pages:	str. 131-137
Volume:	ǂLetn. ǂ46
Issue:	ǂšt. ǂ2
Chronology:	mar.-apr. 2012
ID:	9591581