Nanobody technology

principles & applications

Abstract

Antibodies possess unattainable capacity to bind selectively and at high affinity their cognates. For this reason they have been largely used in applications which rely on specific molecular recognition. Biosensors, nanoparticles, and even cells can be functionalized with antibodies for improving sensitivity and target specificity. However, conventional antibodies (IgGs) are large molecules (150 kDa) that are difficult to engineer. In the last years, antibody fragments have become more and more popular as an effective alternative and specifically nanobodies raised enthusiasm because of their minimal mass (14 kDa), high stability, relative similarity to human sequences, and simplified mutagenesis. Pre-immune nanobody libraries have the further advantage of enabling blind selection for antigens that can be used to discriminate between subpopulations of cells and vesicles. The panning can be performed directly on intact cells and the resulting binders are specific for the native antigen conformation

Keywords

Nanobodies;in vitro panning;cancer biomarkers;recombinant antibody engineering;

Data

Language:	English
Year of publishing:	2017
Typology:	3.14 - Invited Lecture at Foreign University
Organization:	UNG - University of Nova Gorica
UDC:	577
COBISS:	4775675
Views:	4233
Downloads:	0
Average score:	0 (0 votes)
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