Separacija bioaktivnih komponent iz brusnic

diplomsko delo

Mojca Peklar (Author), Mojca Škerget (Mentor), Željko Knez (Co-mentor)

Abstract

S pomočjo konvencionalne ekstrakcije in superkritične ekstrakcije s CO2 posušenih plodov brusnic smo pripravili ekstrakte v katerih smo določili vsebnost nekaterih fenolnih spojin. Pri konvencionalni ekstrakciji smo kot topilo uporabili 60 % raztopino acetona in 35 % raztopino etanola. Ekstrakcije smo izvajali pri temperaturi 22 °C in pri temperaturi 40 °C v dveh stopnjah. V pripravljenih ekstraktih smo določali koncentracije celokupnih monomernih antocianinov, celokupnih fenolov, celokupnih flavonoidov, proantocianidinov in taninov s spektrofotometričnimi metodami. Merili smo jim absorbanco pri različnih valovnih dolžinah in na podlagi dobljenih umeritvenih krivulj izračunali koncentracije. Rezultate smo podali na gram ekstrakta in na gram materiala. Prav tako smo spremljali kinetiko ekstrakcije, tako da smo med postopkom ekstrakcije jemali vzorce v različnih časovnih intervalih in spremljali spreminjanje koncentracije v odvisnosti od časa.

Keywords

konvencionalna ekstrakcija;superkritična ekstrakcija;brusnica;celokupni fenoli;celokupni flavonoidi;celokupni antocianini;tanini;proantocianidini;kinetika ekstrakcije;

Data

Language:	Slovenian
Year of publishing:	2009
Source:	Maribor
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[M. Peklar]
UDC:	66.061.34:547.568(043.2)
COBISS:	13440022
Views:	3469
Downloads:	438
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Separation of bioactive compounds from cranberries
Secondary abstract:	Dried cranberries were extracted and obtained extracts were analyzed on the content of phenolic compounds. Conventional extraction with organic solvents and supercritical extraction with CO2 were applied. For conventional extraction 60 % solution of acetone and 35 % solution of ethanol were used. Extractions were performed at temperature 22°C and at temperature 40 °C in two steps. Concentrations of total monomeric anthocyanins, total phenols, total flavonoids, proanthocyanidins and tanins were determined in prepared extracts by spectrophotometric methods. The absorbances at different wavelengths were measured and according to calibration curves concentrations were calculated. Results were expressed per gram of extract and per gram of material. The kinetics of extractions was followed, so that the samples were taken at different time intervals during the process of extraction and variation of concentration with time was monitored.
Secondary keywords:	conventional extraction;supercritical extraction;cranberry;total phenols;total flavonoids;total anthocianins;tanins;proanthocianins;kinetics of extraction;
URN:	URN:SI:UM:
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	XII, 80 f.
Keywords (UDC):	applied sciences;medicine;technology;uporabne znanosti;medicina;tehnika;chemical technology;chemical and related industries;kemijska tehnologija;kemijske in sorodne industrije;chemical technology of liquids;processes and equipment for treatment of or with liquids;kemijska tehnologija tekočin;procesi in oprema za obdelovanje tekočin ali za obdelovanje z njimi;mathematics;natural sciences;naravoslovne vede;matematika;chemistry;crystallography;mineralogy;kemija;organic chemistry;organska kemija;
ID:	986753