diplomsko delo
Tilen Poljšak (Author), Darinka Brodnjak-Vončina (Mentor), Mitja Kolar (Co-mentor), Uroš Gregorc (Co-mentor)

Abstract

Pri proizvodnji zdravil je kakovost izdelka ključna in najpomembnejša, četudi so zahteve po hitrejši in učinkovitejši proizvodnji vse bolj prisotne tudi v farmacevtski industriji. Namen diplomskega dela je dokazati ustreznost izolacije terapevtskega proteina z uporabo afinitetne kromatografije, za potrebe analitike, za dokazovanje njegove ustreznosti. Dva različna terapevtska proteina smo izolirali v več ponovitvah in analizne rezultate primerjali z rezultati dveh referenčnih proteinov. Izsledki analiz kažejo, da je metoda izolacije ustrezna in ne vpliva na strukturo proteina.

Keywords

izolacija;terapevtski proteini;afinitetna kromatografija;

Data

Language: Slovenian
Year of publishing:
Source: Maribor
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [T. Poljšak]
UDC: 543,384.054(043.2)
COBISS: 16034326 Link will open in a new window
Views: 1957
Downloads: 162
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Other data

Secondary language: English
Secondary title: Testing of therapeutic protein isolation using affinity chromatography on the automatic TECAN system
Secondary abstract: Despite the existing demands in the pharmaceutical industry for faster and more efficient production, quality of the final product is crucial in the production of medicinal products. The purpose of the diploma paper is to demonstrate the adequacy of therapeutic protein isolation by using affinity chromatography for needs of analysis. We have isolated two different therapeutic proteins in a number of replicates and compared the results with the results of two referential proteins. These have shown that the isolation methods are appropriate and do not affect the structure of the protein.
Secondary keywords: isolation;therapeutic protein;affinity chromatography;
URN: URN:SI:UM:
Type (COBISS): Undergraduate thesis
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: 34 f.
Keywords (UDC): mathematics;natural sciences;naravoslovne vede;matematika;chemistry;crystallography;mineralogy;kemija;analytical chemistry;analizna kemija;
ID: 994678
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