Določevanje monosaharidov s kapilarno elektroforezo

diplomsko delo univerzitetnega študijskega programa

Natalija Virant (Author), Mitja Kolar (Mentor), Darinka Brodnjak-Vončina (Co-mentor), Vanja Kokol (Co-mentor)

Abstract

Namen diplomskega dela je bil s pomočjo kapilarne elektroforeze kvalitativno in kvantitativno ovrednotiti sestavo monosaharidov v vzorcih kefirana. Optimirali smo celoten postopek z uporabo različnih dolžin kapilare ter različnih dodatkov acetonitrila k boratnemu pufru. Vzorce kefirana smo hidrolizirali s H2SO4, derivatizacijo pa smo izvedli z reduktivno aminacijo, ob uporabi natrijevega cianoborohidrida. Rezultati so pokazali, da kefiran vsebuje glukozo in galaktozo. Določili smo, da je skupna vrednost monosaharidov v območju od 29,2 % do 55,8 %. Koncentracija glukoze je bila v območju od 62,8 mg/L do 113,7 mg/L, medtem ko se je koncentracije galaktoze gibala med 55,2 mg/L in 110,1 mg/L. Ugotovili smo, da je najboljša ločljivost komponent potekla ob uporabi kapilare dolžine 37 cm in 30 % dodatkom acetonitrila k boratnemu pufru.

Keywords

kapilarna elektroforeza;elektroosmotski tok;monosaharidi;

Data

Language:	Slovenian
Year of publishing:	2012
Source:	Maribor
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[N. Virant]
UDC:	543.062:577.114.3(043.2)
COBISS:	16710678
Views:	1814
Downloads:	234
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Determination of mono-saccharides using capillary electrophoresis
Secondary abstract:	The purpose of my diploma thesis was to use capillary electrophoresis in order to evaluate the qualitative and quantitative composition of mono-saccharides in the kefiran samples. We optimized the whole process by using capillary of different lengths and different additions of acetonitrile to the borate buffer. Kefiran samples were hydrolysed by sulphuric acid, and derivatization was performed by reductive amination using sodium cyanoborohydride. The results showed that kefiran contains glucose and galactose. We determined that the total value of monosaccharide was in the range from 29,2 % to 55,8 %. The glucose concentration was in the range from 62,8 mg/L to 113,7 mg/L, while the concentrations of galactose ranged from 55,2 mg/L to 110,1 mg/L. We found that the best resolution of components was established by using a 37 cm long capillary and 30 % acetonitrile addition to the borate buffer.
Secondary keywords:	capillary;electroosmotic flow;mono-saccharides;kefiran;
URN:	URN:SI:UM:
Type (COBISS):	Bachelor thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	IX, 43 str.
Keywords (UDC):	mathematics;natural sciences;naravoslovne vede;matematika;chemistry;crystallography;mineralogy;kemija;analytical chemistry;analizna kemija;mathematics;natural sciences;naravoslovne vede;matematika;biological sciences in general;biologija;material bases of life;biochemistry;molecular biology;biophysics;biokemija;molekularna biologija;biofizika;
ID:	997138