diplomsko delo univerzitetnega študijskega programa
Katja Golob (Author), Mojca Škerget (Mentor), Mateja Primožič (Co-mentor)

Abstract

S predhodno termično obdelavo in liofilizacijo smo stabilizirali vzorce. Z metodo konvencionalne šaržne ekstrakcije smo pripravili ekstrakte iz različno obdelanih vzorcev rdeče pese. Ekstrakcijo smo izvajali pri konstantnem mešanju na magnetnem mešalu, pri sobni temperaturi, zavarovano pred vdorom dnevne svetlobe, dve uri. Vzporedno smo analizirali tudi vzorce neekstrahiranega materiala rdeče pese. Vzorce smo v daljšem časovnem obdobju izpostavljali različnim procesnim parametrom, kot sta svetloba in sobna temperatura. Določali smo koncentracije celokupnih fenolov, celokupnih flavonoidov in antioksidativno aktivnost po dveh metodah (β-karotensko in radikalsko metodo) ter procentualno razmerje betalainov (razmerja betaksantinov in vulgaksantinom v rdeči pesi) smo določevali s pomočjo spektrofotometričnih metod. Kinetiko razpada oz. spreminjanja koncentracije s časom, smo določevali s pomočjo metode inkubacije vzorcev pri različnih temperaturah, in sicer pri temperaturah 20 ËšC, 40 °C in 60 °C. Kinetiko smo spremljali tako, da smo v različnih časovnih intervalih merili absorbance raztopin vzorcev pri absorpcijskih maksimumih, pri valovnih dolžinah λmax530 nm in λmax480 nm.

Keywords

konvencionalna ekstrakcija;rdeča pesa;celokupni fenoli;celokupni flavonoidi;antioksidativna aktivnost;

Data

Language: Slovenian
Year of publishing:
Source: Maribor
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [K. Golob]
UDC: 66.061.3(043.2)
COBISS: 14931990 Link will open in a new window
Views: 3531
Downloads: 209
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Other data

Secondary language: English
Secondary title: Phenolic compounds and betalains from beetroot and kinetics of degradation
Secondary abstract: Beetroot samples were extracted and fresh material was prepaired. The obtained extracts and samples of material were analized on contant of phenolic compaunds. Used was a conventional extraction with constant mixing. With destilated H2O as solvent, the extraction duration of 2 hours and shield against the effects of daylight. Thermic treatment and liofilization was used to stabilize obtaind compaunds. The samples of beetroot were exposed to differend process parameters. The concentrations of total phenols, total flavonoids, total monomeric anthocyanins, antioksidative activity and the percentage of betalains were determined in the prepared samples by spectrophotometrical metods. The kinetics of degradation was followed by chattering the samples at differend temperatures (20 °C, 40 °C, 60 °C). The kinetics was attended by measuring the detection of absorbances at λmax530 (nm) and λmax480 (nm).
Secondary keywords: conventional extraction;beetroot;total phenols;total flavonoids;antioksidative activity;betalains;kinetics of degradation;
URN: URN:SI:UM:
Type (COBISS): Undergraduate thesis
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: X, 71 f.
Keywords (UDC): applied sciences;medicine;technology;uporabne znanosti;medicina;tehnika;chemical technology;chemical and related industries;kemijska tehnologija;kemijske in sorodne industrije;chemical technology of liquids;processes and equipment for treatment of or with liquids;kemijska tehnologija tekočin;procesi in oprema za obdelovanje tekočin ali za obdelovanje z njimi;
ID: 999210