Ivan Radosavljević (Avtor), Zlatko Šatović (Avtor), Jernej Jakše (Avtor), Branka Javornik (Avtor), Danijela Greguraš (Avtor), Marija Jug-Dujaković (Avtor), Zlatko Liber (Avtor)

Povzetek

Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H$_E$) was compared to the expected genetic diversity at mutation-drift equilibrium (H$_{EQ}$) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.

Ključne besede

salvia;common sage;laminaceae;microsatellites;microsatellite markers;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 577.2:633.88
COBISS: 7252089 Povezava se bo odprla v novem oknu
ISSN: 1661-6596
Št. ogledov: 1065
Št. prenosov: 473
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: navadni žajbelj;dalmatinski žajbelj;mikrosatelitski markerji;mikrosateliti;populacije;laminaceae;genetska pestrost;
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 12082-12093
Letnik: ǂVol. ǂ13
Zvezek: ǂIssue ǂ9
Čas izdaje: 2012
DOI: 10.3390/ijms130912082
ID: 1032524