Effect of base sequence on G-wire formation in solution

Lea Spindler (Avtor), Martin Rigler (Avtor), Irena Drevenšek Olenik (Avtor), Nason Ma'ani Hessari (Avtor), Mateus Webba da Silva (Avtor)

Povzetek

The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating.

Ključne besede

DNA;dynamic light scattering;DLS;polyacrylamide gel electrophoresis;PAGE;genetics;

Podatki

Jezik:	Angleški jezik
Leto izida:	2010
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FS - Fakulteta za strojništvo
UDK:	53
COBISS:	23737127
ISSN:	2090-021X
Matična publikacija:	G-Quadruplex Nucleic Acids
Št. ogledov:	966
Št. prenosov:	328
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	DNK;dinamično sipanje svetlobe;DLS;poliakrilamidna gelska elektroforeza;PAGE;genetika;
URN:	URN:SI:UM:
Vrsta dela (COBISS):	Znanstveno delo
Strani:	str. 431651-1-431651-8
Letnik:	ǂVol. ǂ2010
Čas izdaje:	2010
DOI:	10.4061/2010/431651
ID:	10842665