Separation of peptides with forward osmosis biomimetic membranes

Niada Bajraktari (Avtor), Henrik T. Madsen (Avtor), Mathias F. Gruber (Avtor), Sigurd Truelsen (Avtor), Elzbieta L. Jensen (Avtor), Henrik Jensen (Avtor), Claus Hélix-Nielsen (Avtor)

Povzetek

Forward osmosis (FO) membranes have gained interest in several disciplines for the rejection and concentration of various molecules. One application area for FO membranes that is becoming increasingly popular is the use of the membranes to concentrate or dilute high value compound solutions such as pharmaceuticals. It is crucial in such settings to control the transport over the membrane to avoid losses of valuable compounds, but little is known about the rejection and transport mechanisms of larger biomolecules with often flexible conformations. In this study, transport of two chemically similar peptides with molecular weight (Mw) of 375 and 692 Da across a thin film composite Aquaporin Inside™ Membrane (AIM) FO membrane was investigated. Despite the relative large size, both peptides were able to permeate the dense active layer of the AIM membrane and the transport mechanism was determined to be diffusion-based. Interestingly, the membrane permeability increased 3.65 times for the 692 Da peptide (1.39 × 10−12 m2·s−1) compared to the 375 Da peptide (0.38 × 10−12 m2·s−1). This increase thus occurs for an 85% increase in Mw but only for a 34% increase in peptide radius of gyration (Rg) as determined from molecular dynamics (MD) simulations. This suggests that Rg is a strong influencing factor for membrane permeability. Thus, an increased Rg reflects the larger peptide chains ability to sample a larger conformational space when interacting with the nanostructured active layer increasing the likelihood for permeation.

Ključne besede

membrane;biomimetične membrane;osmoza;biomimetic membranes;forward osmosis;separation;

Podatki

Jezik:	Angleški jezik
Leto izida:	2016
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK:	66.02
COBISS:	21185046
ISSN:	2077-0375
Št. ogledov:	881
Št. prenosov:	372
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	direktna osmoza;biomimetika;peptidi;zavrnitev;
URN:	URN:SI:UM:
Vrsta dela (COBISS):	Znanstveno delo
Strani:	str. 1-12
Letnik:	ǂVol. ǂ6
Zvezek:	ǂiss. ǂ4
Čas izdaje:	2016
DOI:	10.3390/membranes6040046
ID:	10843819