ǂa ǂpilot study
Andrea Quagliariello (Avtor),
Irene Aloisio (Avtor),
Nicole Bozzi Cionci (Avtor),
Donata Luiselli (Avtor),
Giuseppe D'Auria (Avtor),
Llúcia Martinez-Priego (Avtor),
David Pérez-Villarroya (Avtor),
Tomaž Langerholc (Avtor),
Maša Primec (Avtor),
Dušanka Mičetić-Turk (Avtor),
Diana Di Gioia (Avtor)
Povzetek
Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.
Ključne besede
coeliac disease;gluten free diet;probiotic;Bifidobacterium breve;intestinal microbiota;qPCR;next generation sequencing;
Podatki
Jezik: |
Angleški jezik |
Leto izida: |
2016 |
Tipologija: |
1.01 - Izvirni znanstveni članek |
Organizacija: |
UM FKBV - Fakulteta za kmetijstvo in biosistemske vede |
UDK: |
579 |
COBISS: |
4240940
|
ISSN: |
2072-6643 |
Št. ogledov: |
1444 |
Št. prenosov: |
167 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Slovenski jezik |
Sekundarne ključne besede: |
celiakija;brezglutenska dieta;probiotik;Bifidobacterium breve;črevesna mikrobiota;qPCR;verižna reakcija s polimerazo;sekvenciranje nove generacije;NGS; |
URN: |
URN:SI:UM: |
Vrsta dela (COBISS): |
Znanstveno delo |
Strani: |
16 str. (660) |
Letnik: |
ǂVol. ǂ8 |
Zvezek: |
ǂIss. ǂ10 |
Čas izdaje: |
2016 |
DOI: |
10.3390/nu8100660 |
ID: |
10844036 |