16S rRNA in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production

Janja Trček (Avtor), Luka Lipoglavšek (Avtor), Gorazd Avguštin (Avtor)

Povzetek

Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has oft en been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production.

Ključne besede

mikrobiologija;ocetnokislinske bakterije;pretočna citometrija;microbiology;acetic acid bacteria;flow cytometry;

Podatki

Jezik:	Angleški jezik
Leto izida:	2016
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FNM - Fakulteta za naravoslovje in matematiko
UDK:	579.66
COBISS:	22612232
ISSN:	1330-9862
Št. ogledov:	27226
Št. prenosov:	325
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	mikrobiologija;ocetnokislinske bakterije;pretočna citometrija;
URN:	URN:SI:UM:
Vrsta dela (COBISS):	Znanstveno delo
Strani:	str. 108-112
Letnik:	ǂVol. ǂ54
Zvezek:	ǂno. ǂ1
Čas izdaje:	2016
DOI:	10.17113/ftb.54.01.16.4041
ID:	10850643