Ronan Crepin (Avtor), Ario De Marco (Avtor)

Povzetek

Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.

Ključne besede

nanobodies;GPCR proteins;FSHR;panning;pre-immune antibody library;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UNG - Univerza v Novi Gorici
UDK: 577
COBISS: 4933627 Povezava se bo odprla v novem oknu
ISSN: 0006-291X
Št. ogledov: 3319
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

URN: URN:SI:UNG
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: str. 1567-1572
Letnik: ǂVol. ǂ493
Zvezek: ǂiss. ǂ4
Čas izdaje: 2017
DOI: 10.1016/j.bbrc.2017.10.036
ID: 10871790
Priporočena dela:
, ǂa ǂuniversal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
, lecture at the Faculty of Chemistry, University of Belgrade, 22. 9. 2016
, lecture at Institut für Pharmazeutische und Medizinische Chemie, Westfälische Wilhelms-Universität Münster, 10. 5. 2017
, lecture at Department of Biochemistry of Technische Universität Darmstadt, 12. 9. 2018