diplomsko delo
Povzetek
Na mikroalgah osnovane biotehnološke platforme zaenkrat še niso enakovredne
ostalim platformam, ki se uporabljajo v biotehnologiji (bakterije, kvasovke, rastlinske
in živalske celične kulture, rastline itd.). Za svojo rast v težkih pogojih proizvajajo
razne metabolite, ki so zanimivi tudi za prehrambno industrijo in zdravstvo. Z vidika
transformacije pa so zanimivi predvsem kloroplasti, ki so značilni za rastlinske celice
in cianobakterije ter imajo velik biotehnološki potencial. Zaradi svojega razvoja, iz
prej samostojnih prokariontskih organizmov, je njihov genom (plastom) manj
kompleksen in ga lažje modificiramo. Ta metoda transformacije plastoma se uporablja
za pridobivanje širokega spektra rekombinantnih proteinov in tudi drugih spojin. S
homologno rekombinacijo se v plastom lahko vstavi gene sorodnih mikroalg ali drugih
organizmov. Vstavi se lahko enega ali več genov, spreminja metabolne poti, ki so že
prisotne v kloroplastih. V diplomskem delu so predstavljene tudi možne slabosti
transformacije kloroplastov ter predlogi za izboljšave. Na dveh primerih je prikazana
uspešna transformacija in produkcija industrijsko pomembnih fitaze in astaksantina.
Ključne besede
mikroalge;plastom;Chlamydomonas reinhardtii;industrijski proizvodi;
Podatki
Jezik: |
Slovenski jezik |
Leto izida: |
2021 |
Tipologija: |
2.11 - Diplomsko delo |
Organizacija: |
UL BF - Biotehniška fakulteta |
Založnik: |
[A. Rupar] |
UDK: |
606:602.3:579.871:602.6:582.303/.305:604.4(043.2) |
COBISS: |
68240387
|
Št. ogledov: |
249 |
Št. prenosov: |
25 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Angleški jezik |
Sekundarni naslov: |
Transformation of microalgal chloroplasts to obtain valuable industrial products |
Sekundarni povzetek: |
Microalgae-based biotechnology platforms are not yet equivalent to other platforms
used in biotechnology (bacteria, yeast, plant and animal cell cultures, plants etc.). For
their growth in harsh environments, they produce various metabolites that are
interesting for food industry as well as healthcare. Chloroplasts, which are specific for
plant cells and cyanobacteria, have great biotechnological potential and are
particularly interesting for transformation. Because of their evolution from previously
autonomous prokaryotic organism, their genome (plastome) is less complex and easier
to modify. This method of plastome transformation is used to obtain a wide specter of
recombinant proteins and other compounds. By homologous recombination, genes of
related microalgae or other organisms can be inserted into the plastome. One or more
genes can be inserted, in addition the already present metabolic pathways can be
modified. The diploma thesis also presents possible disadvantages of chloroplast
transformation and suggestions for improvements. Two cases are shown to present
successful transformation and production of industrially important phytase and
astaxantin. |
Sekundarne ključne besede: |
microalgae;industrial products; |
Vrsta dela (COBISS): |
Diplomsko delo/naloga |
Študijski program: |
0 |
Komentar na gradivo: |
Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije |
Strani: |
VI, 20 str. |
ID: |
13069929 |