magistrsko delo
Povzetek
V nalogi smo z metodo slikovne citometrije analizirali velikost jedrnega genoma
kačnikovk, ki smo jih nabrali v La Gambi (Kostarika). Ugotavljali smo, ali imajo rastline z
isto življenjsko obliko podobne velikosti genomov, ali imajo sorodne vrste podobne velikosti
genomov in ali velikost genoma kaže, da je v raziskovanih vzorcih prisotna poliploidija.
Izmerili smo velikost genoma 46 vrst iz 12 rodov, pri čemer smo ugotovili velik razpon
vrednosti 2C. Pridobili smo prve podatke za 39 vrst in 3 rodove. Potrdili smo, da življenjska
oblika raziskovanih vrst ni povezana z velikostjo genoma, obstaja pa povezava med
velikostjo genoma in življenjsko dobo. Vrste v ožjem sorodstvu so imele podobne velikosti
genomov le pri rodovih Philodendron in Pistia. Poliploidijo smo ovrgli pri taksonu
Philodendron sp. (vzorec Etl #9), za vrsto Philodendron cf. sulcatum pa bi potrebovali
dodatne raziskave. Prešteli smo število kromosomov nedoločene vrsterodu Adelonema (2n
= 10), kar je prvi podatek za ta rod. Za 5 vrst priporočamo dodatne meritve zaradi
metodoloških pomankljivosti, ostale meritve pa so primerne za vnos v spletno bazo vrednosti
C (Royal Botanic Gardens, Kew, VB). Naše meritve smo primerjali z znanimi podatki
kačnikovk, enokaličnic in kritosemenk na splošno ter ugotovili, da imajo kritosemenke v
povprečju manjši genom kot kačnikovke, čeprav so taksonomska nadkategorija, ki vključuje
kačnikovke in enokaličnice.
Ključne besede
slikovna citometrija;genom;kačnikovke;
Podatki
Jezik: |
Slovenski jezik |
Leto izida: |
2021 |
Tipologija: |
2.09 - Magistrsko delo |
Organizacija: |
UL BF - Biotehniška fakulteta |
Založnik: |
[D. Kocjan] |
UDK: |
581.1(043.2) |
COBISS: |
71911171
|
Št. ogledov: |
297 |
Št. prenosov: |
37 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Angleški jezik |
Sekundarni naslov: |
Determining the amount of nuclear DNA of selected tropical species in the Arum family (Araceae) |
Sekundarni povzetek: |
We measured nuclear genome size of aroids, collected in La Gamba (Costa Rica),
using DNA image cytometry. We aimed to establish whether plants with the same life form
have similar genome size, whether closely related species have similar genome sizes and
whether polyploidy is present in any of the studied samples. We measured 46 species from
12 genera, in which we found a wide range in 2C-values. 39 species and 3 genera were
measured for the first time. We determined that life form is not correlated with genome size,
while life cycle is. Closely related species had similar genome sizes only in genera
Philodendron and Pistia. Polyploidy was disproved for taxon Philodendron sp. (sample Etl
#9), while additional research is necessary for Philodendron cf. sulcatum. We also counted
the chromosome number for an unidentified species of genus Adelonema (2n = 10), which
is the first data for this genus. Due to methodological flaws, we recommend additional
measurements for 5 species, while other measurements could be included into the online C-
value database by Royal Botanic Gardens, Kew (Great Britain). Our measurements were
compared with known data of aroids, monocots, and angiosperms in general. We found
angiosperms have on average a smaller genome size than aroids, despite being the clade that
includes both aroids and monocots. |
Sekundarne ključne besede: |
image cytometry;genome size;aroids; |
Vrsta dela (COBISS): |
Magistrsko delo/naloga |
Študijski program: |
0 |
Konec prepovedi (OpenAIRE): |
1970-01-01 |
Komentar na gradivo: |
Univ. Ljubljana, Biotehniška fak. |
Strani: |
IX, 56, [1] f. |
ID: |
13137073 |