Galina V Beznoussenko (Avtor), Sergei S. Pilyugin (Avtor), Willie J. C. Geerts (Avtor), Michael M. Kozlov (Avtor), Koert N. J. Burger (Avtor), Alberto Luini (Avtor), Jure Derganc (Avtor), Alexander A. Mironov (Avtor)

Povzetek

Membrane organelles often have complicated shapes and differ in their volume, surface area and membrane curvature. The ratio between the surface area of the cytosolic and luminal leaflets (trans-membrane area asymmetry (TAA)) determines the membrane curvature within different sites of the organelle. Thus, the shape of the organelle could be critically dependent on TAA. Here, using mathematical modeling and stereological measurements of TAA during fast transformation of organelle shapes, we present evidence that suggests that when organelle volume and surface area are constant, TAA can regulate transformation of the shape of the Golgi apparatus, endosomal multivesicular bodies, and microvilli of brush borders of kidney epithelial cells. Extraction of membrane curvature by small spheres, such as COPI-dependent vesicles within the Golgi (extraction of positive curvature), or by intraluminal vesicles within endosomes (extraction of negative curvature) controls the shape of these organelles. For instance, Golgi tubulation is critically dependent on the fusion of COPI vesicles with Golgi cisternae, and vice versa, for the extraction of membrane curvature into 50-60 nm vesicles, to induce transformation of Golgi tubules into cisternae. Also, formation of intraluminal ultra-small vesicles after fusion of endosomes allows equilibration of their TAA, volume and surface area. Finally, when microvilli of the brush border are broken into vesicles and microvilli fragments, TAA of these membranes remains the same as TAA of the microvilli. Thus, TAA has a significant role in transformation of organelle shape when other factors remain constant.

Ključne besede

lipidna membrana;asimetrija;Golgijev aparat;lipid membrane;asymmetry;Golgi apparatus;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 577
COBISS: 32179417 Povezava se bo odprla v novem oknu
ISSN: 1422-0067
Št. ogledov: 0
Št. prenosov: 1
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Neznan jezik
Sekundarne ključne besede: lipidna membrana;asimetrija;Golgijev aparat;
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: str. 5299-5333
Letnik: ǂVol. ǂ16
Zvezek: ǂiss. ǂ3
Čas izdaje: Mar. 2015
DOI: 10.3390/ijms16035299
ID: 13424958