Identification and validation of new cancer stem cell-related genes and their regulatory microRNAs in colorectal cancerogenesis

Kristian Urh (Avtor), Margareta Žlajpah (Avtor), Nina Zidar (Avtor), Emanuela Boštjančič (Avtor)

Povzetek

Significant progress has been made in the last decade in our understanding of the pathogenetic mechanisms of colorectal cancer (CRC). Cancer stem cells (CSC) have gained much attention and are now believed to play a crucial role in the pathogenesis of various cancers, including CRC. In the current study, we validated gene expression of four genes related to CSC, L1TD1, SLITRK6, ST6GALNAC1 and TCEA3, identified in a previous bioinformatics analysis. Using bioinformatics, potential miRNA-target gene correlations were prioritized. In total, 70 formalin-fixed paraffin-embedded biopsy samples from 47 patients with adenoma, adenoma with early carcinoma and CRC without and with lymph node metastases were included. The expression of selected genes and microRNAs (miRNAs) was evaluated using quantitative PCR. Differential expression of all investigated genes and four of six prioritized miRNAs (hsa-miR-199a-3p, hsa-miR-335-5p, hsa-miR-425-5p, hsa-miR-1225-3p, hsa-miR-1233-3p and hsa-miR-1303) was found in at least one group of CRC cancerogenesis. L1TD1, SLITRK6, miR-1233-3p and miR-1225-3p were correlated to the level of malignancy. A negative correlation between miR-199a-3p and its predicted target SLITRK6 was observed, showing potential for further experimental validation in CRC. Our results provide further evidence that CSC-related genes and their regulatory miRNAs are involved in CRC development and progression and suggest that some them, particularly miR-199a-3p and its SLITRK6 target gene, are promising for further validation in CRC.

Ključne besede

colorectal cancer;differentially expressed genes;cancer stem cells;

Podatki

Jezik:	Angleški jezik
Leto izida:	2021
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL MF - Medicinska fakulteta
UDK:	616-006
COBISS:	53489155
ISSN:	2227-9059
Št. ogledov:	137
Št. prenosov:	51
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	kolorektalni rak;različno izraženi geni;matične celice raka;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-22
Letnik:	ǂVol. ǂ9
Zvezek:	ǂiss. ǂ2
Čas izdaje:	2021
DOI:	10.3390/biomedicines9020179
ID:	14562744