Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)

Sandra Ropret (Avtor), Jakob Jeriha (Avtor), Tjaša Sorčan (Avtor), Marija Rogar (Avtor), Špela Zemljič Jokhadar (Avtor), John A. McGrath (Avtor), Dusko Ilic (Avtor), Mirjana Liović (Avtor)

Povzetek

We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, plu-ripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi- 004A line has been also tested for ability to differentiate into fibroblasts.

Ključne besede

matične celice;epidermoliza;kolagen VII;stem cell;epidermolysis;collagen VII;

Podatki

Jezik:	Angleški jezik
Leto izida:	2021
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL MF - Medicinska fakulteta
UDK:	61
COBISS:	74980611
ISSN:	1876-7753
Št. ogledov:	52
Št. prenosov:	29
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	matične celice;epidermoliza;kolagen VII;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-5
Zvezek:	ǂVol. ǂ55
Čas izdaje:	Jul. 2021
DOI:	10.1016/j.scr.2021.102463
ID:	16261714