magistrsko delo
Ana Gubenšek (Avtor), Tadeja Matos (Recenzent), Polona Zalar (Mentor)

Povzetek

Proteus anguinus ali človeška ribica je endemični močeril Dinarskega krasa, ki je zaradi svoje ogroženosti na rdečem seznamu Svetovne zveze za varstvo narave. Zaradi svoje ekološke niše je izjemno občutljiv na okužbe s patogeni. Večina raziskav se zaradi nedostopnosti podzemeljskega okolja osredotoča na živali v ujetništvu, v tej nalogi pa poročamo o raznovrstnosti gojljivih gliv izoliranih s kože živali iz narave (20 živali) in ujetništva (22 živali) ter njihovega neposrednega vodnega okolja. Kožo živali smo na neinvaziven način vzorčili z brisi, v podzemna vodna telesa pa smo za različna časovna obdobja nastavili keratinske vabe in vzorčeno vodo filtrirali ter inkubirali na različnih gojiščih. Skupaj smo pridobili 392 glivnih izolatov (239 s kože in 153 iz vode), ki smo jih morfološko in molekularno identificirali do skupno 85 različnih rodov in 154 glivnih vrst. Tako s kože bolnih kot zdravih živali smo izolirali večinoma po en izolat in več različnih vrst, vendar so prevladovale filamentozne glive in je bila mikrobna raznolikost večja pri zdravih živalih. Na koži smo zasledili prisotnost oportuno patogenih gliv, povzročiteljice kromomikoz in mukoromikoz pri dvoživkah, in sicer vrste iz rodov Acremonium, Aspergillus, Cladosporium, Exophiala, Fusarium, Mucor, Ochroconis, Phialophora in Penicillium. Glive v vodnem okolju se razlikujejo od kožnega mikobioma živali, saj smo v vodi z uporabljenimi tehnikami zasledili predvsem vrste iz rodov Trichoderma, Fusarium in Mucor. V vodi in na eni živali iz narave smo identificirali povzročiteljice saprolegnioz, oomicete Saprolegnia parasitica, S. delica in S. ferax.

Ključne besede

človeška ribica;Proteus anguinus;kožni mikrobiom;glive;oportunistični patogeni;Saprolegnia;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UL BF - Biotehniška fakulteta
Založnik: [A. Gubenšek]
UDK: 579.25/.26:582.28:597.92
COBISS: 120941827 Povezava se bo odprla v novem oknu
Št. ogledov: 22
Št. prenosov: 16
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Identification of fungi from cave salamander (Proteus anguinus) skin living in nature and captivity
Sekundarni povzetek: Proteus anguinus is a neotenic cave salamander endemic to the Dinaric Karst and is on the World Conservation Union Red List due to its endangerment. As an endemic species with a narrow ecological niche, the olm is also highly susceptible to infection with water transmitted pathogens. Due to the inaccessibility of the subterranean environment, most scientific studies have focused on captive animals. However, in this master thesis we report on the diversity of cultivable fungi isolated from the skin of animals found in the wild (20 specimens), in captivity (22 specimens), as well as isolated from underground water environment. Animal skin was sampled non-invasively with swabs. Keratin-based baits were placed in the underground water for different time periods, and the water was filtered as well. All samples ware incubated on various media. A total of 392 fungal isolates (239 from skin and 153 from water) were obtained and identified using morphology and molecular data, comprising a total of 85 different genera and 154 species. Both syptomatic and asyptomatic animals were infected with different fungal species, most of which were represented by single isolates. Filamentous fungi were predominant, and microbial diversity was greater in asyptomatic animals. Oportunistic pathogenic fungi were detected, mainly the causative agents of chromomycosis and mucoromycosis in amphibians, from the genera Acremonium, Aspergillus, Cladosporium, Exophiala, Fusarium, Mucor, Ochroconis, Phialophora, and Penicillium. The composition of fungi sampled from the aquatic environment using the applied techniques differed from the cutaneous mycobiome of animals, with the predominant fast-growing fungi of the genera Trichoderma, Fusarium, and Mucor. The causative agents of saprolegniosis, the oomycetes Saprolegnia parasitica, S. delica, and S. ferax, were identified in water and in a symptomatic animal from the wild.
Sekundarne ključne besede: cave salamander;Proteus anguinus;cutaneous mycrobiome;fungi;opportunistic pathogens;Saprolegnia;
Vrsta dela (COBISS): Magistrsko delo/naloga
Študijski program: 0
Konec prepovedi (OpenAIRE): 1970-01-01
Komentar na gradivo: Univ. v Ljubljani, Biotehniška fak., Oddelek za mikrobiologijo
Strani: 1 spletni vir (1 datoteka PDF (XIII, 77 str., [19] f. pril.))
ID: 16420261