Samo Pirnat (Avtor), Mićo Božić (Avtor), Dorian Dolanc (Avtor), Anemari Horvat (Avtor), Petra Tavčar Verdev (Avtor), Nina Vardjan (Avtor), Alexei Verkhratsky (Avtor), Robert Zorec (Avtor), Matjaž Stenovec (Avtor)

Povzetek

The plasticity of astrocytes is fundamental for their principal function, maintaining homeostasis of the central nervous system throughout life, and is associated with diverse exposomal challenges. Here, we used cultured astrocytes to investigate at subcellular level basic cell processes under controlled environmental conditions. We compared astroglial functional and signaling plasticity in standard serum-containing growth medium, a condition mimicking pathologic conditions, and in medium without serum, favoring the acquisition of arborized morphology. Using opto-/electrophysiologic techniques, we examined cell viability, expression of astroglial markers, vesicle dynamics, and cytosolic Ca2+ and cAMP signaling. The results revealed altered vesicle dynamics in arborized astrocytes that was associated with increased resting [Ca2 +]i and increased subcellular heterogeneity in [Ca2+]i, whereas [cAMP]i subcellular dynamics remained stable in both cultures, indicating that cAMP signaling is less prone to plastic remodeling than Ca2+ signaling, possibly also in in vivo contexts.

Ključne besede

astrocytes;vesicles;confocal microscopy;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616-092
COBISS: 73561091 Povezava se bo odprla v novem oknu
ISSN: 0894-1491
Št. ogledov: 32
Št. prenosov: 4
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: astrociti;vezikli;konfokalna mikroskopija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 2899-2916
Letnik: ǂVol. ǂ69
Zvezek: ǂiss. ǂ12
Čas izdaje: 2021
DOI: 10.1002/glia.24076
ID: 18220006