Tjaša Lukan (Avtor), Anže Županič (Avtor), Tjaša Mahkovec Povalej (Avtor), Jacob O. Brunkard (Avtor), Mirjam Kmetič (Avtor), Mojca Juteršek (Avtor), Špela Baebler (Avtor), Kristina Gruden (Avtor)

Povzetek

Hypersensitive response (HR)-conferred resistance is associated with induction of pro-grammed cell death and pathogen spread restriction in its proximity. The exact role of chloro-plastic reactive oxygen species and its link with salicylic acid (SA) signaling in HR remainunexplained. To unravel this, we performed a detailed spatiotemporal analysis of chloroplast redoxresponse in palisade mesophyll and upper epidermis to potato virus Y (PVY) infection in aresistant potato genotype and its transgenic counterpart with impaired SA accumulation andcompromised resistance. Besides the cells close to the cell death zone, we detected individual cells with oxidizedchloroplasts further from the cell death zone. These are rare in SA-deficient plants, suggestingtheir role in signaling for resistance. We confirmed that chloroplast redox changes play impor-tant roles in signaling for resistance, as blocking chloroplast redox changes affected spatialresponses at the transcriptional level. Through spatiotemporal study of stromule induction after PVY infection, we show that stro-mules are induced by cell death and also as a response to PVY multiplication at the front ofinfection. Overall induction of stromules is attenuated in SA-deficient plants.

Ključne besede

chloroplast redox state;hypersensitive response (HR)-conferred resistance;immune signaling;live cell imaging;Solanum tuberosum (potato);spatiotemporal analysis;stromules;virus resistance;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 57
COBISS: 121824771 Povezava se bo odprla v novem oknu
ISSN: 1469-8137
Št. ogledov: 419
Št. prenosov: 149
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: redoks stanje kloroplastov;krompir;preobčutljivostni odgovor;rastlinska imunska signalizacija;
Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 19. 9. 2022;
Strani: str. 548-562
Letnik: ǂVol. ǂ237
Zvezek: ǂiss. ǂ2
Čas izdaje: Jan. 2023
DOI: 10.1111/nph.18425
ID: 18583958