Encapsulation of living photosynthetic organisms in alginate-gelatin hydrogels for controlled cell growth and oxygen production

Tilen Kopač (Avtor), Žan Boček (Avtor), Katarina Petra van Midden (Avtor), Marina Klemenčič (Avtor), Aleš Ručigaj (Avtor)

Povzetek

Alginate-gelatin bicomposite hydrogels have gained prominence in biomedicine and tissue engineering for their biocompatibility, biodegradability, and customizable properties. Since one of the most promising applications of hydrogels is their use as patches for wound healing reliant to oxygen, we prepared alginate-gelatin hydrogels with constant 2% (w/v) alginate and increasing concentration of gelatin (up to 1% (w/v), crosslinked with a solution of 0.43% CaCl$_2$ and used them to encapsulate photosynthetic cells of a unicellular alga Chlamydomonas reinhardtii. We investigated how hydrogel design parameters (crosslink density) influenced cell growth and oxygen production compared to their rheological properties. Our findings demonstrate that optimizing gelatine concentration played an important role in the diffusion of nutrients to the encapsulated cells, the most important parameter in controlling cell growth and oxygen production. We observed that modulating the concentration of gelatin molecules up to the gelation point, which was determined at 0.66% (w/v), significantly impacting nutrient diffusion, cell growth, and oxygen production due to the viscosity effect. Differently, at concentrations of GEL above gelation point (up to 1%) the stable hydrogel network is formed which affect the nutrient diffusion, cell growth, and oxygen production because of crosslink density. The effect of cell concentration on the mechanical properties of the hydrogel was investigated, which are crucial for further development of alginate-gelatin systems with targeted properties for various applications.

Ključne besede

hidrogeli;rast celic;Chlamydomonas reinhardtii;proizvodnja kisika;mehanske lastnosti;difuzijski koeficient hranil;hydrogels;cell growth;oxygen production;mechanical properties;nutrients diffusion coefficient;

Podatki

Jezik:	Angleški jezik
Leto izida:	2023
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK:	66.098
COBISS:	162521347
ISSN:	1369-703X
Št. ogledov:	144
Št. prenosov:	34
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	hidrogeli;rast celic;Chlamydomonas reinhardtii;proizvodnja kisika;mehanske lastnosti;difuzijski koeficient hranil;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-10
Zvezek:	ǂVol. ǂ199, [article no.] 109070
Čas izdaje:	Oct. 2023
DOI:	10.1016/j.bej.2023.109070
ID:	19929341