Jaime Millán-Santiago (Avtor), Rosalba Vitagliano (Avtor), Fortunata Mondella (Avtor), Roberto Mandrioli (Avtor), Roccaldo Sardella (Avtor), Tomaž Vovk (Avtor), Rafael Lucena (Avtor), Soledad Cárdenas (Avtor), Federico Boaron (Avtor), Laura Mercolini (Avtor)

Povzetek

Psychiatric disorders are usually treated with antipsychotic agents belonging to different pharmacological and chemical classes, the most recent ones collectively known as “third-generation antipsychotics”, such as cariprazine, approved in 2015 for the treatment of patients affected by schizophrenia. For these patients, a frequent therapeutic drug monitoring (TDM) becomes essential to assess compliance and to optimise and personalise their therapy, also due to cariprazine interindividual variability and narrow therapeutic range. In this study, a bioanalytical method featuring miniaturised sampling and pretreatment was developed, based on volumetric absorptive microsampling (VAMS) for TDM of psychiatric patients under cariprazine treatment and compared to a classic, reference method based on fluid plasma analysis. Minimally invasive whole blood VAMS was coupled to an original instrumental method based on ultra-high performance liquid chromatography hyphenated to mass spectrometry (UHPLC-MS). A feasible and streamlined, yet reliable VAMS pretreatment protocol was carefully optimised and the VAMS-UHPLC-MS methodology was validated with satisfactory results in terms of linearity (r2 > 0.9970 in the 1.5-100 ng/mL range), precision (%RSD < 11.7), extraction yield (> 90.0%) and matrix effect (RE% 8.2-10.9%). Finally, the microsampling approach coupled to UHPLC-MS was successfully applied to the TDM of psychiatric patients treated with cariprazine and compared with standard fluid plasma analysis, providing reliable quali-quantitative results, and proving to be readily applicable to the clinical practice in TDM programs as a useful alternative to cariprazine plasma analysis. This is the first report of a successful microsampling application, and in particular the first report of VAMS application, for the TDM of cariprazine.

Ključne besede

cariprazine;microsampling;therapeutic drug monitoring (TDM);volumetric absorptive microsampling (VAMS);microsampling (VAMS);

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FFA - Fakulteta za farmacijo
UDK: 615.214:616.89
COBISS: 165592835 Povezava se bo odprla v novem oknu
ISSN: 0731-7085
Št. ogledov: 23
Št. prenosov: 1
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: kariprazin;mikrovzorčenje;terapevtsko spremljanje zdravil;volumetrično absorpcijsko mikrovzorčenje;Antipsihotiki;Psihiatrija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-7
Zvezek: ǂVol. ǂ236
Čas izdaje: 2023
DOI: 10.1016/j.jpba.2023.115740
ID: 20154393