Tatiana Samgina (Avtor), Irina D. Vasileva (Avtor), Polonca Trebše (Avtor), Gregor Torkar (Avtor), Alexey K. Surin (Avtor), Zhaowei Meng (Avtor), Roman Zubarev (Avtor), Albert T. Lebedev (Avtor)

Povzetek

Peptides released on frogs’ skin in a stress situation represent their only weapon against micro-organisms and predators. Every species and even population of frog possesses its own peptidome being appropriate for their habitat. Skin peptides are considered potential pharmaceuticals, while the whole peptidome may be treated as a taxonomic characteristic of each particular population. Continuing the studies on frog peptides, here we report the peptidome composition of the Central Slovenian agile frog Rana dalmatina population. The detection and top-down de novo sequencing of the corresponding peptides was conducted exclusively by tandem mass spectrometry without using any chemical derivatization procedures. Collision-induced dissociation (CID), higher energy collision-induced dissociation (HCD), electron transfer dissociation (ETD) and combined MS3 method EThcD with stepwise increase of HCD energy were used for that purpose. MS/MS revealed the whole sequence of the detected peptides including differentiation between isomeric Leu/Ile, and the sequence portion hidden in the disulfide cycle. The array of the discovered peptide families (brevinins 1 and 2, melittin-related peptides (MRPs), temporins and bradykinin-related peptides (BRPs)) is quite similar to that of R. temporaria. Since the genome of this frog remains unknown, the obtained results were compared with the recently published transcriptome of R. dalmatina.

Ključne besede

agile brown frog;membrane-active peptides;tandem mass spectrometry;top-down de novo sequencing;bradykinin-related peptides;BRP;EThcD;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL PEF - Pedagoška fakulteta
UDK: 543.51
COBISS: 168907267 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 64
Št. prenosov: 3
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: okretna rjava žaba;membransko aktivni peptidi;tandemska masna spektrometrija;de novo sekvenciranje od zgoraj navzdol;bradikininu sorodni peptidi;BRP;EThcD;
Vrsta dela (COBISS): Članek v reviji
Strani: 14 str.
Letnik: ǂVol. ǂ28
Zvezek: ǂissue ǂ20, [article no.] 7118
Čas izdaje: Oct. 2023
DOI: 10.3390/molecules28207118
ID: 20369638