Daniela Milosheska (Avtor), Robert Roškar (Avtor)

Povzetek

The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl–Hexyl column at 40 °C, using methanol and potassium phosphate buffer (25 mM; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1–50 mg/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide; 0.5–50 mg/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine; 0.2–10 mg/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide; 0.1–10 mg/L for oxcarbazepine; 2–100 mg/L for felbamate and 3–150 mg/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy.

Ključne besede

antiepileptična zdravila;človeška plazma;terapevtsko spremljanje zdravil;validacija bioanalitskih metod;tekočinska kromatografija visoke ločljivosti;antiepileptic drugs;human plasma;therapeutic drug monitoring;bioanalytical method validation;high-performance liquid chromatography;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FFA - Fakulteta za farmacijo
UDK: 543.544.5:616.853
COBISS: 174900739 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 6
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Tekočinska kromatografija;Epilepsija;
Vrsta dela (COBISS): Članek v reviji
Strani: 15 str.
Letnik: ǂVol. ǂ28
Zvezek: ǂiss. ǂ23, [article no.] 7830
Čas izdaje: 2023
DOI: 10.3390/molecules28237830
ID: 21465712