Žiga Jelen (Avtor), Janez Kovač (Avtor), Rebeka Rudolf (Avtor)

Povzetek

This study reports on the successful conjugation of SARS-CoV-2 S1 spike protein fragments with gold nanoparticles (AuNPs) that were synthesised with Ultrasonic Spray Pyrolysis (USP). This method enables the continuous synthesis of AuNPs with a high degree of purity, round shapes, and the formation of a surface that allows various modifications. The conjugation mechanism of USP synthesized AuNPs with SARS-CoV-2 S1 spike protein fragments was investigated. A gel electrophoresis experiment confirmed the successful conjugation of AuNPs with SARS-CoV-2 S1 fragments indirectly. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed the presence of characteristic O1s and N1s peaks, which indicated that specific binding between AuNPs and SARS-CoV-2 S1 spike protein fragments takes place via a peptide bond formed with the citrate stabiliser. This bond is coordinated to the AuNP’s surface and the N-terminals of the protein, with the conjugate displaying the expected response within a prototype LFIA test. This study will help in better understanding the behaviour of AuNPs synthesised with USP and their potential use as sensors in colorimetric or electrochemical sensors and LFIA tests.

Ključne besede

nanodelci zlata;ultrazvočna razpršilna piroliza;konjugacija;SARS-CoV-2 S1;karakterizacija;gold nanoparticles;ultrasonic spray pyrolysis;conjugation;characterisation;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FS - Fakulteta za strojništvo
Založnik: MDPI
UDK: 669.2:620.3
COBISS: 159776515 Povezava se bo odprla v novem oknu
ISSN: 2079-4991
Št. ogledov: 204
Št. prenosov: 9
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: nanodelci zlata;ultrazvočna razpršilna piroliza;konjugacija;SARS-CoV-2 S1;karakterizacija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-14
Letnik: ǂVol. ǂ13
Zvezek: ǂiss. ǂ15, [article no.] 2160
Čas izdaje: Aug. 2023
DOI: 10.3390/nano13152160
ID: 21520813