Nada Elmerhi (Avtor), Khadega Al-Maqdi (Avtor), Khawlah Athamneh (Avtor), Abdul Khayum Mohammed (Avtor), Tina Škorjanc (Avtor), Felipe Gándara (Avtor), Jesus Raya (Avtor), Pascal Simon (Avtor), Olivier Siri (Avtor), Ali Trabolsi (Avtor)

Povzetek

Efficient enzyme immobilization is crucial for the successful commercialization of large-scale enzymatic water treatment. However, issues such as lack of high enzyme loading coupled with enzyme leaching present challenges for the widespread adoption of immobilized enzyme systems. The present study describes the development and bioremediation application of an enzyme biocomposite employing a cationic macrocycle-based covalent organic framework (COF) with hierarchical porosity for the immobilization of horseradish peroxidase (HRP). The intrinsic hierarchical porous features of the azacalix[4]arene-based COF (ACA-COF) allowed for a maximum HRP loading capacity of 0.76 mg/mg COF with low enzyme leaching (<5.0%). The biocomposite, HRP@ACA-COF, exhibited exceptional thermal stability (~200% higher relative activity than the free enzyme), and maintained ~60% enzyme activity after five cycles. LCMSMS analyses confirmed that the HRP@ACA-COF system was able to achieve >99% degradation of seven diverse types of emerging pollutants (2-mercaptobenzothiazole, paracetamol, caffeic acid, methylparaben, furosemide, sulfamethoxazole, and salicylic acid)in under an hour. The described enzyme-COF system offers promise for efficient wastewater bioremediation applications.

Ključne besede

covalent organic frameworks;enzymes;emerging pollutants;water purification;biocomposite;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UNG - Univerza v Novi Gorici
UDK: 54
COBISS: 161219843 Povezava se bo odprla v novem oknu
ISSN: 0304-3894
Št. ogledov: 463
Št. prenosov: 2
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Vrsta dela (COBISS): Delo ni kategorizirano
Strani: str. 1-9
Letnik: ǂVol. ǂ459
Zvezek: [article no.] 132261
Čas izdaje: Oct. 2023
DOI: 10.1016/j.jhazmat.2023.132261
ID: 21843022