Microfluidic electrochemical glucose biosensor with in situ enzyme immobilization

Nina Lokar (Avtor), Borut Pečar (Avtor), Matej Možek (Avtor), Danilo Vrtačnik (Avtor)

Povzetek

The development and characterization of a microfluidic electrochemical glucose biosensor are presented herein. The transducer part is based on thin-film metal electrodes on a glass substrate. The biological recognition element of the biosensor is the pyrroloquinoline quinone–glucose dehydrogenase (PQQ-GdhB) enzyme, selectively in situ immobilized via microcontact printing of a mixed self-assembling monolayer (SAM) on a gold working electrode, while the microfluidic part of the device comprises microchannel and microfluidic connections formed in a polydimethylsiloxane (PDMS) elastomer. The electrode properties throughout all steps of biosensor construction and the biosensor response to glucose concentration and analyte flow rate were characterized by cyclic voltammetry and chronoamperometry. A measurement range of up to 10 mM in glucose concentration with a linear range up to 200 µM was determined. A detection limit of 30 µM in glucose concentration was obtained. Respective biosensor sensitivities of 0.79 nA/µM/mm$^2$ and 0.61 nA/µM/mm$^2$ were estimated with and without a flow at 20 µL/min. The developed approach of in situ enzyme immobilization can find a wide number of applications in the development of microfluidic biosensors, offering a path towards continuous and time-independent detection.

Ključne besede

mikrofluidni biosenzor glukoze;elektrokemični biosenzor;tankoplastna elektroda;imobilizacija encima in situ;kronoamperometrija;ciklična voltamerija;microfluidic glucose biosensor;electrochemical biosensor;thin film electrode;enzyme in situ immobilization;chronoamperometry;cyclic voltammetry;

Podatki

Jezik:	Angleški jezik
Leto izida:	2023
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL FE - Fakulteta za elektrotehniko
UDK:	602.1:53.082.9
COBISS:	145284355
ISSN:	2079-6374
Št. ogledov:	37
Št. prenosov:	7
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	mikrofluidni biosenzor glukoze;elektrokemični biosenzor;tankoplastna elektroda;imobilizacija encima in situ;kronoamperometrija;ciklična voltametrija;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-16
Letnik:	ǂVol. ǂ13
Zvezek:	ǂiss. ǂ3, [article no.] 364
Čas izdaje:	Mar. 2023
DOI:	10.3390/bios13030364
ID:	22583478