Validation of interferometric light microscopy for assessment of extracellular particles in 250 samples of diluted plasma

preparing the path for future clinical practices

Maxence Berry (Avtor), Matevž Arko (Avtor), Anna Romolo (Avtor), Maja Brložnik (Avtor), Anita Mrvar Brečko (Avtor), Boštjan Korenjak (Avtor), Aleš Iglič (Avtor), Vesna Kadunc Kos (Avtor), Peter Kruljc (Avtor), Alenka Nemec Svete (Avtor), Vladimira Erjavec (Avtor), Veronika Kralj-Iglič (Avtor)

Povzetek

In recent years, the analysis of extracellular particles (EPs) has become instrumental in deciphering intercellular communication and disease biomarkers. Interferometric light microscopy (ILM) has emerged as a powerful label-free technique for real-time characterization of nanoscale particles. This contribution presents for the first time measurements of EPs directly in diluted plasma by ILM on populations of samples. The study involves the measured number density of EPs, the corrected number density of EPs (taking into account dilution of the sample) and the size of EPs. Analysis involves plasma of multiple species—canine, equine, and human. Most of the D$_h$ in all three species were confined to the interval between 130 nm and 200 nm. We found no statistically significant correlation between the corrected n and D$_h$ indicating good performance of the method. The correlation between the measured n and D$_h$ was statistically significant indicating hindered movement of EPs due to their higher number density. These results showed that high throughput measurement of number density and size of EPs in plasma is feasible.

Ključne besede

extracellular vesicles;interferometric light microscopy;plasma analysis;nanoparticle characterization;videodrop technology;nanoparticles;

Podatki

Jezik:	Angleški jezik
Leto izida:	2024
Tipologija:	1.08 - Objavljeni znanstveni prispevek na konferenci
Organizacija:	UL ZF - Zdravstvena fakulteta
UDK:	577
COBISS:	188588547
Št. ogledov:	333
Št. prenosov:	51
Ocena:	0 (0 glasov)
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