Megan H. Cleveland (Avtor), Hua-Jun He (Avtor), Mojca Milavec (Avtor), Young-Kyung Bae (Avtor), Peter M. Vallone (Avtor), Jim F. Huggett (Avtor)

Povzetek

Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials.

Ključne besede

digitalna PCR;digitalna verižna reakcija s polimerazo;referenčni materiali;UV-spektroskopija;digital PCR;dPCR;reference materials;UV-spectroscopy;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.02 - Pregledni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 577.2
COBISS: 186484995 Povezava se bo odprla v novem oknu
ISSN: 1872-9452
Št. ogledov: 30
Št. prenosov: 19
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: digitalna PCR;digitalna verižna reakcija s polimerazo;referenčni materiali;UV-spektroskopija;
Konec prepovedi (OpenAIRE): 2025-02-14
Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 22. 2. 2024;
Strani: str. 1-8
Letnik: ǂVol. ǂ96
Zvezek: [article no.] 101256
Čas izdaje: 2024
DOI: 10.1016/j.mam.2024.101256
ID: 23984520