Hana Esih (Avtor), Klemen Mezgec (Avtor), Martina Billmeier (Avtor), Špela Malenšek (Avtor), Mojca Benčina (Avtor), Blaž Grilc (Avtor), Duško Lainšček (Avtor), Roman Jerala (Avtor), Sara Vidmar (Avtor), Mirjana Gašperlin (Avtor), Marjan Bele (Avtor), Mihaela Zidarn (Avtor), Tatjana Lejko-Zupanc (Avtor), Tina Morgan (Avtor)

Povzetek

The delivery of vaccines plays a pivotal role in influencing the strength and longevity of the immune response and controlling reactogenicity. Mucosal immunization, as compared to parenteral vaccination, could offer greater protection against respiratory infections while being less invasive. While oral vaccination has been presumed less effective and believed to target mainly the gastrointestinal tract, trans-buccal delivery using mucoadhesive films (MAF) may allow targeted delivery to the mucosa. Here we present an effective strategy for mucosal delivery of several vaccine platforms incorporated in MAF, including DNA plasmids, viral vectors, and lipid nanoparticles incorporating mRNA (mRNA/LNP). The mRNA/LNP vaccine formulation targeting SARS-CoV-2 as a proof of concept remained stable within MAF consisting of slowly releasing water-soluble polymers and an impermeable backing layer, facilitating enhanced penetration into the oral mucosa. This formulation elicited antibody and cellular responses comparable to the intramuscular injection, but also induced the production of mucosal IgAs, highlighting its efficacy, particularly for use as a booster vaccine and the potential advantage for protection against respiratory infections. The MAF vaccine preparation demonstrates significant advantages, such as efficient delivery, stability, and simple noninvasive administration with the potential to alleviate vaccine hesitancy.

Ključne besede

vaccine delivery;mucoadhesive film;mucosal immunity;respiratory infection;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FFA - Fakulteta za farmacijo
UDK: 615.371
COBISS: 197189635 Povezava se bo odprla v novem oknu
ISSN: 0168-3659
Št. ogledov: 111
Št. prenosov: 22
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: dostava cepiv;mukoadhezivni film;imuniteta sluznice;okužba dihal;sproščanje;erozija;Imunski odziv;Dostavni sistemi;Cepiva;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 179-192
Zvezek: ǂVol. ǂ371
Čas izdaje: 2024
DOI: 10.1016/j.jconrel.2024.05.041
ID: 24298690