Hana Majaron (Avtor), Boštjan Kokot (Avtor), Anja Pišlar (Avtor), Hana Esih (Avtor), Alen Gabrič (Avtor), Dunja Urbančič (Avtor), Rojbin El (Avtor), Iztok Urbančič (Avtor), Stane Pajk (Avtor)

Povzetek

Plasma membranes are vital biological structures, serving as protective barriers and participating in various cellular processes. In the field of super-resolution optical microscopy, stimulated emission depletion (STED) nanoscopy has emerged as a powerful method for investigating plasma membrane-related phenomena. However, many applications of STED microscopy are critically restricted by the limited availability of suitable fluorescent probes. This paper reports on the development of two amphiphilic membrane probes, SHE-2H and SHE-2N, specially designed for STED nanoscopy. SHE-2N, in particular, demonstrates quick and stable plasma membrane labelling with negligible intracellular redistribution. Both probes exhibit outstanding photostability and resolution improvement in STED nanoscopy, and are also suited for two-photon excitation microscopy. Furthermore, microscopy experiments and cytotoxicity tests revealed no noticeable cytotoxicity of probe SHE-2N at concentration used for fluorescence imaging. Spectral analysis and fluorescence lifetime measurements conducted on probe SHE-2N using giant unilamellar vesicles, revealed that emission spectra and fluorescence lifetimes exhibited minimal sensitivity to lipid composition variations. These novel probes significantly augment the arsenal of tools available for high-resolution plasma membrane research, enabling a more profound exploration of cellular processes and dynamics

Ključne besede

fizika;membrane;fluorescenca;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: IJS - Institut Jožef Stefan
UDK: 539
COBISS: 198949635 Povezava se bo odprla v novem oknu
ISSN: 0045-2068
Št. ogledov: 61
Št. prenosov: 39
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: fizika;membrane;fluorescenca;
Vrsta dela (COBISS): Članek v reviji
Strani: 10 str.
Letnik: ǂVol. ǂ150
Zvezek: ǂArt. ǂ107554
Čas izdaje: 2024
DOI: 10.1016/j.bioorg.2024.107554
ID: 24373235