Borut Šketa (Avtor), James L. Galman (Avtor), Nicholas J. Turner (Avtor), Polona Žnidaršič Plazl (Avtor)

Povzetek

Enzyme immobilization is one of the essential tools that enables bioprocess intensification as it facilitates reuse of enzyme. Nanomaterials can be used as supports for enzyme immobilization because of their high surface to volume ratio. In his work a designed biocompatible non-woven nanofiber was used as a support for hexahistidine tagged amine transaminase immobilization in a microreactor. Four different nanofibers were tested and the one with surface immobilized Cu$^{2+}$ ions was proven to be the best. Additionally, direct immobilization of overexpressed enzymes in E. coli cell lysate was performed and yielded loads up to 1088 U mL$^{-1}$. The highest turnover number reached within the indicated time was 7.23 10$^6$.

Ključne besede

imobilizacija encimov;amin transaminaze;mikroreaktor;membrana;enzyme immobilization;amine transaminase;microreactor;membrane;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 66.098
COBISS: 200710147 Povezava se bo odprla v novem oknu
ISSN: 1871-6784
Št. ogledov: 53
Št. prenosov: 16
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: imobilizacija encimov;amin transaminaze;mikroreaktor;membrana;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 46-55
Zvezek: ǂVol. ǂ83
Čas izdaje: 25 Nov. 2024
DOI: 10.1016/j.nbt.2024.06.005
ID: 24524837