Katja Kološa (Avtor), Aleš Leskovšek (Avtor), Teja Rajar (Avtor), Tamara Lah Turnšek (Avtor)

Povzetek

The major obstacle to the application of mesenchymal stromal cells (MSCs) in regenerative medicine is the expansion of the donor-derived cells in vitro to obtain high cell numbers in the shortest possible time. However, MSCs gradually undergo replicative senescence after a variable number of divisions that reduce their therapeutic efficacy, which needs to be determined before administration. The authors developed a fast and simple evaluation assay testing two senescence inducers, mitoxantrone (Mxt) and trichostatin A (TSA), to predict the onset of spontaneous replicative senescence of adipose-derived mesenchymal stromal cells (ASCs) and have confirmed the correlation between induced senescence and spontaneous replicative senescence in the assay using Mxt. This protocol facilitates the standardization of therapeutic ASCs and MSCs from other origins before application.

Ključne besede

adipose mesenchymal stromal (stem) cells (ASCs);cell longevity;induced senescence;mesenchymal stromal (stem) cells (MSCs);mitoxantrone;replicative senescence;trichostatin A;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 577.2
COBISS: 100663043 Povezava se bo odprla v novem oknu
ISSN: 0736-6205
Št. ogledov: 1
Št. prenosov: 1
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: maščobne matične celice;dolgoživost celic;mitoksantron;replikativno staranje;trihostatin A;
Strani: str. 1-10
Letnik: ǂVol. ǂ72
Zvezek: ǂno. ǂ3
Čas izdaje: 2022
DOI: 10.2144/btn-2021-0087
ID: 24536889