Marko Petek (Avtor), Anna Coll Rius (Avtor), Rok Ferenc (Avtor), Jaka Razinger (Avtor), Kristina Gruden (Avtor)

Povzetek

Colorado potato beetle (CPB) is an agricultural pest of solanaceous crops, notorious for its rapid resistance development to chemical pesticides. Foliar spraying of dsRNA formulations is a promising innovative technology providing highly specific and environmentally acceptable option for CPB management. We designed dsRNA to silence CPB mesh gene (dsMESH) and performed laboratory feeding trials to assess impacts on beetle survival and development. We compared the effectiveness of in vivo and in vitro produced dsRNA in a series of laboratory experiments. We additionally performed a field trial in which the efficacy of dsRNA sprayed onto potato foliage was compared to a spinosad-based insecticide. We showed that dsMESH ingestion consistently and significantly impaired larval growth and decreased larval survival in laboratory feeding experiments. In vivo produced dsRNA performed similarly as in vitro synthesized dsRNA in laboratory settings. In the field trial, dsMESH was as effective in controlling CPB larvae as a commercial spinosad insecticide, its activity was however slower. We discuss limitations and benefits of a potential dsMESH-based CPB management strategy and list some important RNAi based CPB research topics, which will have to be addressed in future.

Ključne besede

Leptinotarsa decemlineata;E. coli;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 632
COBISS: 25419523 Povezava se bo odprla v novem oknu
ISSN: 1664-462X
Št. ogledov: 319
Št. prenosov: 127
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarne ključne besede: krompirjev hrošč;koloradski hrošč;škodljvci;poljski poskusi;laboratorijski poskusi;RNA interferenca;dvovijačna RNA;zatiranje škodljivcev z RNAi;analiza preživetja;
Komentar vira: Nasl. iz nasl. zaslona; Opis vira z dne 18.8.2020; ǂarticle no.ǂ1250;
Strani: Str. 1-9
Letnik: ǂVol. ǂ11
Zvezek: ǂarticle no.ǂ1250
Čas izdaje: 2020
DOI: 10.3389/fpls.2020.01250
ID: 24574219