Vid Jazbec (Avtor), Nina Varda (Avtor), Ernest Šprager (Avtor), Maja Meško (Avtor), Sara Vidmar (Avtor), Rok Romih (Avtor), Marjetka Podobnik (Avtor), Andreja Kežar (Avtor), Roman Jerala (Avtor), Mojca Benčina (Avtor)

Povzetek

Gas vesicles (GVs) are large cylindrical gas-filled protein assemblies found in diverse aquatic bacteria that enable their adaptation of buoyancy. GVs have already been used as ultrasound contrasting agents. Here, we investigate GVs derived from Bacillus megaterium, aiming to minimize the number of accessory Gvps within the GV gene cluster and demonstrate the use of GVs as enhancers of acoustic radiation force administered by ultrasound. Three (GvpR, GvpT, and GvpU) out of 11 genes in the cluster were found to be dispensable for functional GV formation, and their omission resulted in narrower GVs. Two essential proteins GvpJ and GvpN were absent from recently determined GV structures, but GvpJ was nevertheless found to be tightly bound to the cylindrical part of GVs in this study. Additionally, the N-terminus of GvpN was observed to play an important role in the formation of mature GVs. The binding of engineered GvpC from Anabaena flos-aquae to HEK293 cells via integrins enhanced the acoustic force delivered by ultrasound and resulted in an increased Ca$^{2+}$ influx into cells. Coupling with a synthetic Ca$^{2+}$-dependent signaling pathway GVs efficiently enhanced cell stimulation by ultrasound, which expands the potentials of noninvasive sonogenetics cell stimulation.

Ključne besede

biokemija;beljakovine;proteini;plinski vezikli;bakterije;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: KI - Kemijski inštitut
Založnik: American Chemical Society
UDK: 577
COBISS: 203392515 Povezava se bo odprla v novem oknu
ISSN: 1936-086X
Št. ogledov: 18
Št. prenosov: 4
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: biokemija;beljakovine;proteini;plinski vezikli;bakterije;
Vrsta dela (COBISS): Članek v reviji
Komentar vira: Nasl. z nasl. zaslona; Opis vira z dne 1. 8. 2024;
Strani: str. 16692–16700
Letnik: ǂVol. ǂ18
Zvezek: ǂiss. ǂ26
Čas izdaje: Jul. 2, 2024
DOI: 10.1021/acsnano.4c01498
ID: 24798259