Boštjan Kokot (Avtor), Hana Majaron (Avtor), Polona Umek (Avtor), Katarina Petra van Midden (Avtor), Stane Pajk (Avtor), Maja Garvas (Avtor), Christian Eggeling (Avtor), Tilen Koklič (Avtor), Iztok Urbančič (Avtor), Janez Štrancar (Avtor)

Povzetek

Nanotechnologies hold great promise for various applications. To predict and guarantee the safety of novel nanomaterials, it is essential to understand their mechanism of action in an organism, causally connecting adverse outcomes with early molecular events. This is best investigated using noninvasive advanced optical methods, such as high-resolution live-cell fluorescence microscopy, which require stable labeling of nanoparticles with fluorescent dyes. However, as shown here, when the labeling is performed inadequately, unbound fluorescent dyes and inadvertently altered chemical and physical properties of the nanoparticles can result in experimental artefacts and erroneous conclusions. To prevent such unintentional errors, we introduce a tested minimal combination of experimental methods to enable artefact-free fluorescent labeling of metal-oxide nanoparticles—the largest subpopulation of nanoparticles by industrial production and applications—and demonstrate its application in the case of TiO2 nanotubes. We (1) characterize potential changes of the nanoparticles’ surface charge and morphology that might occur during labeling by using zeta potential measurements and transmission electron microscopy, respectively, and (2) assess stable binding of the fluorescent dye to the nanoparticles with either fluorescence intensity measurements or fluorescence correlation spectroscopy, which ensures correct nanoparticle localization. Together, these steps warrant the reliability and reproducibility of advanced optical tracking, which is necessary to explore nanomaterials’ mechanism of action and will foster widespread and safe use of new nanomaterials.

Ključne besede

dye leakage;labeling characterization;artefacts;labeling-induced changes;reliable nanomaterial labeling;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FNM - Fakulteta za naravoslovje in matematiko
Založnik: Informa Healthcare
UDK: 53
COBISS: 79746819 Povezava se bo odprla v novem oknu
ISSN: 1743-5404
Št. ogledov: 21
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: uhajanje barvila;karakterizacija označevanja;artefakti;spremembe zaradi označevanja;zanesljivo označevanje nanomaterialov;
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 1102-1123
Letnik: ǂVol. ǂ15
Zvezek: ǂno. ǂ8
Čas izdaje: 2021
DOI: 10.1080/17435390.2021.1973607
ID: 24824357