Jan Kejžar (Avtor), Polona Mrak (Avtor), Ilja Gasan Osojnik Črnivec (Avtor), Nataša Poklar Ulrih (Avtor)

Povzetek

We investigated the influence of archaeal lipids (C25,25) isolated from thermophilic archaeon Aeropyrum pernix K1 on physicochemical properties of liposomes comprised of egg sphingomyelin (SM) and cholesterol (CH) using fluorescence emission anisotropy, calcein release studies, dynamic light scattering, transmission electron microscopy and phase analysis light scattering. The 2 mol% addition of archaeal lipids enabled formation of small unilamellar vesicles by sonication while also having significant effect on reducing mean size, polydispersity index and zeta potential of C25,25/SM/CH vesicles. Increasing the ratio of C25,25 lipids in mixture of C25,25/SM/CH decreased lipid ordering parameter in dose dependent manner at different temperatures. We also demonstrated that adding 15 mol% C25,25 to SM/CH mixture will cause it to notably interact with fetal bovine serum which could make them a viable alternative adjuvant to synthetic ether-linked lipids in development of advanced liposomal vaccine delivery systems. The prospect of combining the proven strengths of SM/CH mixtures with the unique properties of C25,25 opens exciting possibilities for advancing drug delivery technologies, promising to yield formulations that are both highly effective and adaptable to a range of therapeutic applications.

Ključne besede

liposomi;lipidi arhej;sfingomielin;holesterol;fizikalnokemijske lastnosti;dostavni sistemi;liposomes;archaeal lipids;sphingomyelin;cholesterol;membrane fluidity;drug delivery;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 577.35:582.233:615.014.6
COBISS: 205727235 Povezava se bo odprla v novem oknu
ISSN: 0005-2736
Št. ogledov: 25
Št. prenosov: 4
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: liposomi;lipidi arhej;sfingomielin;holesterol;fizikalnokemijske lastnosti;dostavni sistemi;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-9
Letnik: ǂVol. ǂ1866
Zvezek: ǂissue ǂ7, [article no.] 184374
Čas izdaje: Oct. 2024
DOI: 10.1016/j.bbamem.2024.184374
ID: 24866560