Maruša Ramšak (Avtor), Dominique A. Ramirez (Avtor), Loren E. Hough (Avtor), Michael R. Shirts (Avtor), Sara Vidmar (Avtor), Kristina Eleršič (Avtor), Gregor Anderluh (Avtor), Roman Jerala (Avtor)

Povzetek

Membraneless liquid compartments based on phase-separating biopolymers have been observed in diverse cell types and attributed to weak multivalent interactions predominantly based on intrinsically disordered domains. The design of liquid-liquid phase separated (LLPS) condensates based on de novo designed tunable modules that interact in a well-understood, controllable manner could improve our understanding of this phenomenon and enable the introduction of new features. Here we report the construction of CC-LLPS in mammalian cells, based on designed coiled-coil (CC) dimer-forming modules, where the stability of CC pairs, their number, linkers, and sequential arrangement govern the transition between diffuse, liquid and immobile condensates and are corroborated by coarse-grained molecular simulations. Through modular design, we achieve multiple coexisting condensates, chemical regulation of LLPS, condensate fusion, formation from either one or two polypeptide components or LLPS regulation by a third polypeptide chain. These findings provide further insights into the principles underlying LLPS formation and a design platform for controlling biological processes.

Ključne besede

biokemija;biopolimeri;biološki procesi;celice;sesalci;simulacije;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: KI - Kemijski inštitut
UDK: 577
COBISS: 177039875 Povezava se bo odprla v novem oknu
ISSN: 2041-1723
Št. ogledov: 202
Št. prenosov: 146
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: biokemija;biopolimeri;biološki procesi;celice;sesalci;simulacije;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-15
Letnik: ǂVol. ǂ14
Zvezek: article no. 7973
Čas izdaje: 2023
DOI: 10.1038/s41467-023-43742-w
ID: 25381353