M.P. Romero (Avtor), L. Calvo (Avtor), C. Alba (Avtor), Maja Leitgeb (Avtor), Mateja Primožič (Avtor), Željko Knez (Avtor)

Povzetek

Isoamyl acetate was successfully synthesized from isoamyl alcohol in supercritical carbon dioxide by enzymatic catalysis. First, the impact of the acyl donor was investigated. Among several reactants, including acetic acid and two different acetates, acetic anhydride gave best yields. Then, two different immobilized lipases (Novozym 435 from Candida antarctica and Lipozyme RM-IM from Rhizomucor miehei) as biocatalysts for the above-mentionedreaction were compared. An esterification extent of 100% was obtained in continuous operation using acetic anhydride as acyl donor and Novozym 435 as enzyme. The amount of enzyme preparation was optimised to 6.25 g/mol alcohol. The effect of substrates load in the solvent was investigated. Operating at a CO2žsubstrates molar ratio below 7.0, the conversion of alcoholdecreased, probably due to an inhibitory effect on enzyme by high concentration of acetic anydride or by produced acetic acid. Pressure in the range of 8-30 MPa showed no effect on this reaction, while an increase in temperature (over 313 K) led to lower production of isoamyl acetate. Novozym 435 was very stable not finding any loss of activity during one month of continuous operation. Finally, carbon dioxide was compared to a conventional organic solvent (n-hexane). Initial reaction rate was higher in SC-CO2, although final esterification extent was similar in both media.

Ključne besede

kemijska procesna tehnika;visokotlačna tehnologija;superkritični CO2;sinteze estrov;izoamil acetat;encimska kataliza;imobilizirana lipaza;proizvodnja izoamil acetata;proizvodnja naravnih arom;chemical processing;high pressure technology;superkritical CO2;ester synthesis;isoamyl acetate;enzymatic catalysis;immobilized lipase;isoamyl acetate production;natural flavors;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 66.091-987
COBISS: 9162774 Povezava se bo odprla v novem oknu
ISSN: 0896-8446
Št. ogledov: 1724
Št. prenosov: 175
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarne ključne besede: kemijska procesna tehnika;visokotlačna tehnologija;superkritični CO2;sinteze estrov;izoamil acetat;encimska kataliza;imobilizirana lipaza;proizvodnja izoamil acetata;proizvodnja naravnih arom;
URN: URN:SI:UM:
Strani: str. 77-84
Letnik: ǂVol. ǂ33
Zvezek: ǂiss. ǂ1
Čas izdaje: 2005
ID: 8718457