Discovery of novel and known viruses associated with toxigenic and non-toxigenic bloom forming diatoms from the Northern Adriatic Sea

Timotej Turk Dermastia (Avtor), Denis Kutnjak (Avtor), Ion Gutierrez-Aguirre (Avtor), Corina P. D. Brussaard (Avtor), Katarina Bačnik (Avtor)

Povzetek

Algal blooms impact trophic interactions, community structure and element fluxes. Despite playing an important role in the demise of phytoplankton blooms, only few viruses infecting diatoms have been cultured. Pseudo-nitzschia is a widespread diatom genus that commonly blooms in coastal waters and contains toxin-producing species. This study describes the characterization of a novel virus infecting the toxigenic species Pseudo-nitzschia galaxiae isolated from the northern Adriatic Sea. The ssRNA virus PnGalRNAV has 29.5 nm ± 1.2 nm icosahedral virions and a genome size of 8.8 kb. It belongs to the picorna-like Marnaviridae family and shows high specificity for P. galaxiae infecting two genetically and morphologically distinct strains. We found two genetically distinct types of this virus and screening of the global virome database revealed matching sequences from the Mediterranean region and China, suggesting its global distribution. Another virus of similar shape and size infecting Pseudo-nitzschia calliantha was found, but its genome could not be determined. In addition, we have obtained and characterized a new virus that infects Chaetoceros tenuissimus. The replicase protein of this virus is very similar to the previously described ChTenDNAV type-II virus, but it has a unique genome and infection pattern. Our study is an important contribution to the collective diatom virus culture collection and will allow further investigation into how these viruses control diatom bloom termination, carbon export and toxin release in the case of Pseudo-nitzschia.

Ključne besede

cvetenje alg;diatomeje;virus;genom;Jadransko morje;morska virologija;ekologija fitoplanktona;morska biologija;algal blooms;diatoms;PnGalRNAV;genome;Adriatic Sea;marine virology;phytoplankton ecology;marine biology;

Podatki

Jezik:	Angleški jezik
Leto izida:	2024
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	NIB - Nacionalni inštitut za biologijo
UDK:	574
COBISS:	212651523
ISSN:	1568-9883
Št. ogledov:	47
Št. prenosov:	20
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	Fitoplankton;
Komentar vira:	Ostali soavtorji: Denis Kutnjak, Ion Gutierrez-Aguirre, Corina P. D. Brussaard, Katarina Bačnik; Online first: 15 Oct. 2024; Članek v PDF formatu obsega 44 str.;
Strani:	str. 1-13
Letnik:	ǂVol. ǂ139
Zvezek:	ǂ[art. no.] ǂ102737
Čas izdaje:	Nov. 2024
DOI:	10.1016/j.hal.2024.102737
ID:	25378448