magistrsko delo
Monika Šelekar (Avtor), Hrvoje Petković (Recenzent), Romana Marinšek-Logar (Mentor), Lijana Fanedl (Komentor)

Povzetek

Povečana proizvodnja mleka in mlečnih izdelkov je prispevala k hitri rasti sirarske industrije, kar vodi do produkcije velikih količin kisle sirotke. Kisla sirotka predstavlja okolijski izziv zaradi visoke organske obremenitve, hkrati pa tudi potencialen vir za pridobivanje obnovljive energije in drugih produktov. Namen magistrske naloge je bil preučiti trajnostno uporabo odpadne kisle sirotke, delno deproteinizirane kisle sirotke in permeata po ultrafiltraciji delno deproteinizirane kisle sirotke. Izvedli smo dva sklopa poskusov. Prvi sklop sta predstavljala testa biometanskega potenciala v laboratorijskih steklenicah, drugi sklop pa poskusa v laboratorijskih bioreaktorjih. Prvi poskus v bioreaktorjih smo izvedli brez dohranjevanja, drugi poskus pa z dohranjevanjem s kislo sirotko in permeatom. V času izvedbe poskusov nismo zaznali zaviranja procesa metanogeneze. Največ bioplina in posledično metana se je proizvedlo pri uporabi substratov kisle sirotke in permeata. V prvem testu biometanskega potenciala so poskusne mešanice s permeatom proizvedle 13 % več metana kot mešanice s kislo sirotko, medtem ko je bila pri ponovitvi testa proizvodnja metana pri permeatu 3,5 % manjša kot pri kisli sirotki. V laboratorijskih bioreaktorjih smo ugotovili, da je pri prvem poskusu poraba kisle sirotke privedla do 28 % večje proizvodnje metana kot permeat, pri drugem poskusu pa je bila ta razlika 18,7 %. Z analizo profilov polimorfizma dolžin končnih restrikcijskih fragmentov smo želeli ugotoviti, ali različne frakcije kisle sirotke vplivajo na profil mikrobioma. Dohranjevanje z kislo sirotko in permeatom je povzročilo spremembe v bakterijskem delu mikrobioma, vendar pa ni vplivalo na sestavo arhejskega dela mikrobioma. Ugotovili smo, da so kisla sirotka, delno deproteinizirana kisla sirotka in permeat mogoče uporabiti za proizvodnjo bioplina.

Ključne besede

kisla sirotka;bioplin;metan;biometanski potencial;TRFLP;struktura mikrobioma;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UL BF - Biotehniška fakulteta
Založnik: [M. Šelekar]
UDK: 606:628.336.6:637.344
COBISS: 235467267 Povezava se bo odprla v novem oknu
Št. ogledov: 31
Št. prenosov: 5
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Evaluation of the biomethane potential from waste acid whey after removal of selected proteins
Sekundarni povzetek: Increased milk and dairy product production has contributed to the rapid growth of the cheese industry, leading to the generation of large quantities of acid whey. Acid whey presents an environmental challenge due to its high organic load, but it is also a potential source for the production of renewable energy and other valuable products.The aim of this master's thesis was to investigate the sustainable use of waste acid whey, partially deproteinized acid whey, and permeate obtained through ultrafiltration of partially deproteinized acid whey. Two sets of experiments were carried out. The first set consisted of biochemical methane potential tests in laboratory bottles, while the second set included experiments in laboratory bioreactors. The first bioreactor experiment was conducted without feeding, while in the second experiment feedingof acid whey and permeate was included. During the experiments, no inhibition of the methanogenesis process was observed. The highest biogas and methane production was achieved using acid whey and permeate as substrates. In the first biochemical methane potential test, mixtures with permeate produced 13% more methane than those with acid whey, whereas in the repeated test, methane production from permeate was 3.5% lower than that from acid whey. In laboratory bioreactors, the use of acid whey in the first experiment resulted in 28% higher methane production compared to permeate, while in the second experiment, this difference was 18.7%. Through terminal restriction fragment length polymorphism analysis, we aimed to determine whether different acid whey fractions influence the microbiome profile. Feeding with acid whey and permeate led to changes in the bacterial community of the microbiome but did not affect the composition of the archaeal community. Our findings indicate that acid whey, partially deproteinized acid whey, and permeate can be used for biogas production.
Sekundarne ključne besede: acid whey;biogas;methane;biomethane potential;TRFLP;microbiome;
Vrsta dela (COBISS): Magistrsko delo/naloga
Študijski program: 0
Konec prepovedi (OpenAIRE): 1970-01-01
Komentar na gradivo: Univ. v Ljubljani, Biotehniška fak., Oddelek za mikrobiologijo
Strani: 1 spletni vir (1 datoteka PDF (XII, 66 str., [3] f. pril.))
ID: 26366405