Vesna Weingerl (Author), Matija Strlič (Author), Drago Kočar (Author)

Abstract

Phenolic antioxidants are usually grouped into flavonoids and non-flavonoids, according to their structure. With regard to the tannic character, phenolic antioxidants are further subdivided to tannic phenols and non-tannic phenols. Collectively, these compounds contribute to the high antioxidant capacity of wine. In this work, we compare determination of gallic acid, catechin, epicatechin, resveratrol, quercetin, dihydrobenzoic acid, sinapic acid, vanillic acid, caffeic acid, chlorogenic acid, ferullic acid, ellagic acid, p-coumaric acid and caftaric acid in 141 wine samples using two liquid chromatographic methods and detection systems, i.e. with UV detection and mass-spectrometric detection. In addition, we applied the conventional Folin-Ciocalteu spectrophotometric method for determination of the total phenolic content in wine samples and compared the results with those obtained using the chromatographic methods. Despite satisfactory correlations statistically significant differences between HPLC-UV/VIS and LC/MS/MS were established, which could be related to coelution not detectable with UV/VIS detectors. The correlations between results of the spectrophotometric method and sum of LC/MS/MS determinations are not satisfactory and are different for white, red, and rosé wines.

Keywords

analizna kemija;biotehnologija;analize živil;vino;antioksidanti;antioksidantske aktivnosti;določevanje polifenolnih antioksidantov v vinu;kromatografska analiza;tekočinska kromatografija visoke ločljivosti;HPLC;UV/VIS detekcija;masnospektrometrična detekcija;spektrofotometrija;polifenoli;food analysis;wine;antioxidants;chromatography;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: Slovensko kemijsko društvo
UDC: 543.544.5.068.7:543.48:663.21
COBISS: 30810373 Link will open in a new window
ISSN: 1318-0207
Views: 1291
Downloads: 112
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Other data

Secondary language: Slovenian
Secondary abstract: Glede na kemijsko sestavo delimo fenolne antioksidante na flavonoide in neflavonoide. Glede na taninski značaj jih delimo na taninske in netaninske fenole. Omenjene fenolne komponente so odgovorne za visok antioksidativni potencial vina. Primerjali smo določanje galne kisline, katehina, epikatehina, resveratrola, kvercetina dihidrobenzojske kisline, sinapinske kisline, vanilinske kisline, kavne kisline, klorogenske kisline, ferulne kisline, elaginske kisline, p-kumarne kisline in kaftarjeve kisline v številnih vzorcih vin z uporabo dveh metod tekočinske kromatografije z UV/VIS detekcijo in z masnospektrometrično detekcijo (LC/MS/MS). Uporabili smo konvencionalno spektrofotometrično metodo s Folin-Ciocalteu-jevim reagentom za določanje vsebnosti skupnih fenolov in rezultate primerjali z rezultati kromatografskih metod. Kljub zadovoljivim korelacijam smo ugotovili statistično signifikantne razlike med HPLC-UV/VIS in LC/MS/MS, kar je lahko posledica koelucije, ki je z UV/VIS detektorjem ne zaznamo. Primerjava med rezultati, dobljenimi s spektrofotometrično metodo in vsoto fenolnih komponent, določanih z LC/MS/MS je pokazala različne korelacijske premice za rdeča, rosé in bela vina.
Secondary keywords: analizna kemija;analitska kemija;biotehnologija;analiza živil;vino;antioksidanti;antioksidantska aktivnost;določevanje polifenolnih antioksidantov v vinu;kromatografska analiza;tekočinska kromatografija visoke ločljivosti;HPLC;UV/VIS detekcija;masnospektrometrična detekcija;spektrofotometrija;polifenoli;
URN: URN:NBN:SI
Type (COBISS): Scientific work
Pages: str. 698-703
Volume: ǂVol. ǂ56
Issue: ǂno. ǂ3
Chronology: 2009
ID: 10859171