magistrsko delo
Ana Bratuša (Author), Irena Ban (Mentor), Uroš Maver (Co-mentor), Janja Stergar (Co-mentor)

Abstract

V okviru magistrskega dela smo s pomočjo sol-gel metode sintetizirali superparamagnetne NiCu nanodelce v matrici silike s sestavo Ni67,5Cu32,5. Po sintezi smo nastale oksidne nanodelce homogenizirali in reducirali do nastanka zlitine NiCu v cevni peči v atmosferi Ar/H2 pri temperaturi 850 °C in času 6 h. Glavni namen magistrskega dela je bila združitev dveh terapevtskih pristopov, to sta magnetna hipertermija in farmakoterapevtsko zdravljenje. V ta namen smo po uspešni sintezi v pore sintetiziranih NiCu nanodelcev v matrici silike vgradili dve zdravilni učinkovini (ZU), benzokain in ketoprofen, ki sta oba znani učinkovini za lajšanje bolečine. Prvi spada med lokalne anestetike (LA), medtem ko drugi spada v skupino nesteroidnih protivnetnih zdravilnih učinkovin (NSAID). Učinkovitost sproščanja obeh uspešno vgrajenih ZU smo ovrednotili s pomočjo in vitro testiranja sproščanja in merjenja absorbance z UV/Vis spektrofotometrom. Pomerjene absorbance smo preračunali v koncentracije oziroma delež sproščenih ZU po določenih časovnih intervalih. Sintetizirane NiCu nanodelce smo pred in po vgradnji zdravilnih učinkovin okarakterizirali in analizirali s pomočjo rentgenske praškovne difrakcije (RTG), Fourier transformirane infrardeče spektroskopije (FTIR), transmisijske elektronske mikroskopije (TEM), termogravimetrične analize (TGA) in magnetnih meritev. S pomočjo modificirane termogravimetrične aparature s permanentnim magnetom smo določili Curiejevo temperaturo sintetiziranih NiCu nanodelcev. Z BET meritvami smo izmerili specifično površino oz. volumen por pred in po vgradnji ZU ter po primerjavi rezultatov ponovno potrdili uspešnost vgradnje obeh zdravilnih učinkovin v pore NiCu nanodelcev v matrici silike.

Keywords

magnetni nanodelci;sol gel metoda;Curierjeva temperatura;sproščanje zdravilne učinkovine;magnetna hipertermija;magistrske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [A. Bratuša]
UDC: 620.3:543.573(043.2)
COBISS: 21049110 Link will open in a new window
Views: 1167
Downloads: 154
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Other data

Secondary language: English
Secondary title: Development of new bioactive coatings integrating active substances on NiCu nanoparticles
Secondary abstract: In this master thesis, we successfully synthesized suparparamagnetic NiCu nanoparticles in a silica matrix using the sol-gel method. The final particle composition was Ni67,5Cu32,5. Following the synthesis, we performed the homogenization and reduction until the formation of the NiCu alloy in the tube furnace in an Ar/H2 atmosphere at a temperature of 850 °C for 6 h. The main aim of the master thesis was to combine two therapeutic approaches, namely magnetic hyperthermia and active pharmacotherapy. For this purpose, two pain reducing drugs, benzocaine and ketoprofene, a local anaestetic (LA) and a nonsteroidal anti-inflammatory drug (NSAID), respectively, were introduced into the pores of the silica coated NiCu nanoparticles. The release performance of both drugs was evaluated using in vitro drug release testing and quantification of the released amount through measurement of the absorbance on a UV/Vis spectrophotometer. Based on the obtained absorbances we calculated the concentration of active substances at chosen time intervals of the release. We characterized and analyzed the synthesized NiCu nanoparticles before and after the introduction of active substances using X-ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and magnetic measurements. With the termogravimetric apparatus upgraded with a permanent magnet, we determined the Curie temperature of the synthesized NiCu nanoparticles. With the BET measurement we measured the specific surface area/volume of pores before and after the introduction of the active substances. Through comparison of the obtained results we confirmed the successful introduction of active substances into pores of the silica on NiCu nanoparticles.
Secondary keywords: nanoparticles;sol-gel method;Curie temperature;drug release;magnetic hyperthermia;
URN: URN:SI:UM:
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: XI, 58 str.
ID: 10859465