magistrsko delo
Zala Ciglar (Author), Urban Švajger (Mentor), Urban Švajger (Thesis defence commission member), Mojca Narat (Thesis defence commission member), Peter Dovč (Thesis defence commission member)

Abstract

Mezenhimske matične celice MSC so celice, ki nam v kliniki omogočajo zdravljenje različnih poškodb in bolezni. Zaradi svoje imunosupresivnosti, so pomemben dejavnik pri zdravljenju bolezni presadka proti gostitelju GvHD, ki lahko nastane po alogenski transplantaciji tkiva. Iz buffy coata, suspenzije levkocitov, granulocitov in nekaj eritrocitov, smo izolirali imunske celice PBMC dveh različnih darovalcev. Ustvarili smo mešano limfocitno reakcijo, ki smo ji dodali po pet različnih koncentracij s 30 Gy obsevanih MSC. Reagent timidin se je vgradil v imunske celice in na podlagi količine, ki jo izmerimo, sklepamo na proliferacijo PBMC celic. Z njo smo ugotavljali inhibitorni učinek MSC na imunske celice. Optimalno število MSC je bilo 10⁵, če bi bila večja, bi prišlo do kontaktne inhibicije. Proliferacija z višanjem števila MSC premosorazmerno pada.

Keywords

mezenhimske matične celice;PBMC;imunosupresivnost;proliferacija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Z. Ciglar]
UDC: 602.9:611.018:57.083.3(043.2)
COBISS: 9008505 Link will open in a new window
Views: 843
Downloads: 296
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Other data

Secondary language: English
Secondary title: Introduction of a test to determine the immunosupressive function of mesenchymal stem cells using a mixed lymphocyte reaction
Secondary abstract: Mesenchymal stem cells MSC are cells, which help us in treating various injuries and deseases in clinic. Because of their immunosupressiveness, they are important factor in GvHD treatment, which may occur after allogenic tissue transplantation. We isolated immune cells PBMC from two different donors. Isolation was made from the buffy coat, a suspension of leukocytes, granulocytes and still some erytrocytes. We created a mixed lymphocyte reaction, where we aded five different concentration of 30 Gy irradiated MSC. The thymidine reagent, which was used, was incorporated into the immune cells. From an amount which was mesured, we concluded proliferation of PBMC. With proliferation, we detected an inhibitory effect of MSC to immune cells. Optimal MSC number was 10⁵. In the case of higher quantity, there would be a contact inhibition, which would cause cells to die. Proliferation with increased MSC quantity, was dropping disproportinately.
Secondary keywords: mesenchymal stem cells;immunosupression;proliferation;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: X, 50 f.
ID: 10944106