magistrsko delo
Zala Ciglar (Avtor), Urban Švajger (Mentor), Urban Švajger (Član komisije za zagovor), Mojca Narat (Član komisije za zagovor), Peter Dovč (Član komisije za zagovor)

Povzetek

Mezenhimske matične celice MSC so celice, ki nam v kliniki omogočajo zdravljenje različnih poškodb in bolezni. Zaradi svoje imunosupresivnosti, so pomemben dejavnik pri zdravljenju bolezni presadka proti gostitelju GvHD, ki lahko nastane po alogenski transplantaciji tkiva. Iz buffy coata, suspenzije levkocitov, granulocitov in nekaj eritrocitov, smo izolirali imunske celice PBMC dveh različnih darovalcev. Ustvarili smo mešano limfocitno reakcijo, ki smo ji dodali po pet različnih koncentracij s 30 Gy obsevanih MSC. Reagent timidin se je vgradil v imunske celice in na podlagi količine, ki jo izmerimo, sklepamo na proliferacijo PBMC celic. Z njo smo ugotavljali inhibitorni učinek MSC na imunske celice. Optimalno število MSC je bilo 10⁵, če bi bila večja, bi prišlo do kontaktne inhibicije. Proliferacija z višanjem števila MSC premosorazmerno pada.

Ključne besede

mezenhimske matične celice;PBMC;imunosupresivnost;proliferacija;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UL BF - Biotehniška fakulteta
Založnik: [Z. Ciglar]
UDK: 602.9:611.018:57.083.3(043.2)
COBISS: 9008505 Povezava se bo odprla v novem oknu
Št. ogledov: 843
Št. prenosov: 296
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Introduction of a test to determine the immunosupressive function of mesenchymal stem cells using a mixed lymphocyte reaction
Sekundarni povzetek: Mesenchymal stem cells MSC are cells, which help us in treating various injuries and deseases in clinic. Because of their immunosupressiveness, they are important factor in GvHD treatment, which may occur after allogenic tissue transplantation. We isolated immune cells PBMC from two different donors. Isolation was made from the buffy coat, a suspension of leukocytes, granulocytes and still some erytrocytes. We created a mixed lymphocyte reaction, where we aded five different concentration of 30 Gy irradiated MSC. The thymidine reagent, which was used, was incorporated into the immune cells. From an amount which was mesured, we concluded proliferation of PBMC. With proliferation, we detected an inhibitory effect of MSC to immune cells. Optimal MSC number was 10⁵. In the case of higher quantity, there would be a contact inhibition, which would cause cells to die. Proliferation with increased MSC quantity, was dropping disproportinately.
Sekundarne ključne besede: mesenchymal stem cells;immunosupression;proliferation;
Vrsta dela (COBISS): Magistrsko delo/naloga
Študijski program: 0
Komentar na gradivo: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Strani: X, 50 f.
ID: 10944106